Tumor scintigraphy by the method for subtracting the initial image with technetium-99m labeled antibody. Export

@article{citeulike:625184, abstract = {The method for subtracting the initial image from the localization image was evaluated for radioimmunoscintigraphy of tumors with technetium-99m (Tc-99m) labeled antibodies. Monoclonal antibodies were parental mouse and mouse-human chimeric antibodies to carcinoembryonic antigen (CEA), designated F11-39 and ChF11-39, respectively, both of which have been found to discriminate CEA in tumor tissues from the CEA-related antigens. After reduction of the intrinsic disulfide bonds, these antibodies were labeled with Tc-99m. In vivo studies were performed on athymic nude mice bearing the human CEA-producing gastric carcinoma xenografts. Though biodistribution results showed selective and progressive accumulation of Tc-99m labeled antibodies at the tumor site, high radioactivity in blood was inappropriate for scintigraphic visualization of the tumors within a few hours. We examined the subtraction of the initial Tc-99m image from the Tc-99m localization image after a few hours. Subtracted images of the same count reflected the in vivo behavior of the Tc-99m radioactivity. The subtracted scintigrams revealed excellent tumor images with no significant extrarenal background. Visualization of the tumor site was dependent on antigen-specific binding and nonspecific exudation. These results demonstrate that a method of subtraction of the initial image may serve as a potentially useful diagnostic method for an abnormal site for agents with a low pharmacokinetic value.}, address = {Faculty of Pharmaceutical Sciences, Fukuoka University, Japan. karube@fukuoka-u.ac.jp}, author = {Karube, Y. and Katsuno, K. and Ito, S. and Matsunaga, K. and Takata, J. and Kuroki, M. and Murakami, M. and Matsuoka, Y.}, citeulike-article-id = {625184}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/10656275}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=10656275}, issn = {0914-7187}, journal = {Ann Nucl Med}, keywords = {antibody, technetium}, month = {December}, number = {6}, pages = {407--413}, posted-at = {2006-05-12 14:55:54}, priority = {0}, title = {Tumor scintigraphy by the method for subtracting the initial image with technetium-99m labeled antibody.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/10656275}, volume = {13}, year = {1999} }

TY - JOUR ID - citeulike:625184 L3 - citeulike-article-id:625184 N2 - The method for subtracting the initial image from the localization image was evaluated for radioimmunoscintigraphy of tumors with technetium-99m (Tc-99m) labeled antibodies. Monoclonal antibodies were parental mouse and mouse-human chimeric antibodies to carcinoembryonic antigen (CEA), designated F11-39 and ChF11-39, respectively, both of which have been found to discriminate CEA in tumor tissues from the CEA-related antigens. After reduction of the intrinsic disulfide bonds, these antibodies were labeled with Tc-99m. In vivo studies were performed on athymic nude mice bearing the human CEA-producing gastric carcinoma xenografts. Though biodistribution results showed selective and progressive accumulation of Tc-99m labeled antibodies at the tumor site, high radioactivity in blood was inappropriate for scintigraphic visualization of the tumors within a few hours. We examined the subtraction of the initial Tc-99m image from the Tc-99m localization image after a few hours. Subtracted images of the same count reflected the in vivo behavior of the Tc-99m radioactivity. The subtracted scintigrams revealed excellent tumor images with no significant extrarenal background. Visualization of the tumor site was dependent on antigen-specific binding and nonspecific exudation. These results demonstrate that a method of subtraction of the initial image may serve as a potentially useful diagnostic method for an abnormal site for agents with a low pharmacokinetic value. IS - 6 JF - Ann Nucl Med SN - 0914-7187 AD - Faculty of Pharmaceutical Sciences, Fukuoka University, Japan. karube@fukuoka-u.ac.jp EP - 413 TI - Tumor scintigraphy by the method for subtracting the initial image with technetium-99m labeled antibody. VL - 13 SP - 407 KW - antibody KW - technetium AU - Karube, Y AU - Katsuno, K AU - Ito, S AU - Matsunaga, K AU - Takata, J AU - Kuroki, M AU - Murakami, M AU - Matsuoka, Y PY - 1999/12// UR - http://view.ncbi.nlm.nih.gov/pubmed/10656275 ER -