Tumor-specific accumulation of 125I-labeled mouse-human chimeric anti-CEA antibody in a xenografted human cancer model demonstrated by whole-body autoradiography and immunostaining. Export

@article{citeulike:625193, abstract = {Whole-body autoradiography (WBAR) was used to study the biodistribution of 125I-labeled mouse-human chimeric antibody (Ch F11-39) to carcinoembryonic antigen (CEA) in athymic nude mice bearing the CEA-producing MKN-45 human gastric carcinoma xenografts. Significantly high uptake of 125I-Ch F11-39 in the tumors obtained by tissue-counting technique was confirmed by WBAR of mice of 12, 24, 48, and 96 h postinjection of 125I-Ch F11-39. When compared with histochemical or immunohistochemical staining results of the tumor tissue sections, imaging profiles of 125I-Ch F11-39 obtained by WBARs were topographically correlated with histopathological findings of tissues and immunohistochemical localization of CEA in the tumor tissues, indicating that the accumulation of 125I-Ch F11-39 at the tumor site is based on its specificity for CEA. These results demonstrate that this chimeric antibody may serve as a potential useful diagnostic and/or therapeutic reagent for human CEA-producing cancers.}, address = {First Department of Biochemistry, School of Medicine, Fukuoka University, Japan.}, author = {Haruno, M. and Kuroki, M. and Matsunaga, K. and Takata, J. and Karube, Y. and Senba, T. and Murakami, M. and Arakawa, F. and Kuwahara, M. and Ozaki, H. and Matsuoka, Y. and Okazaki, M. and Kanda, H.}, citeulike-article-id = {625193}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/8940726}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=8940726}, issn = {0969-8051}, journal = {Nucl Med Biol}, keywords = {antibody, cea}, month = {August}, number = {6}, pages = {821--826}, posted-at = {2006-05-12 14:58:44}, priority = {0}, title = {Tumor-specific accumulation of 125I-labeled mouse-human chimeric anti-CEA antibody in a xenografted human cancer model demonstrated by whole-body autoradiography and immunostaining.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/8940726}, volume = {23}, year = {1996} }

TY - JOUR ID - citeulike:625193 L3 - citeulike-article-id:625193 N2 - Whole-body autoradiography (WBAR) was used to study the biodistribution of 125I-labeled mouse-human chimeric antibody (Ch F11-39) to carcinoembryonic antigen (CEA) in athymic nude mice bearing the CEA-producing MKN-45 human gastric carcinoma xenografts. Significantly high uptake of 125I-Ch F11-39 in the tumors obtained by tissue-counting technique was confirmed by WBAR of mice of 12, 24, 48, and 96 h postinjection of 125I-Ch F11-39. When compared with histochemical or immunohistochemical staining results of the tumor tissue sections, imaging profiles of 125I-Ch F11-39 obtained by WBARs were topographically correlated with histopathological findings of tissues and immunohistochemical localization of CEA in the tumor tissues, indicating that the accumulation of 125I-Ch F11-39 at the tumor site is based on its specificity for CEA. These results demonstrate that this chimeric antibody may serve as a potential useful diagnostic and/or therapeutic reagent for human CEA-producing cancers. IS - 6 JF - Nucl Med Biol SN - 0969-8051 AD - First Department of Biochemistry, School of Medicine, Fukuoka University, Japan. EP - 826 TI - Tumor-specific accumulation of 125I-labeled mouse-human chimeric anti-CEA antibody in a xenografted human cancer model demonstrated by whole-body autoradiography and immunostaining. VL - 23 SP - 821 KW - antibody KW - cea AU - Haruno, M AU - Kuroki, M AU - Matsunaga, K AU - Takata, J AU - Karube, Y AU - Senba, T AU - Murakami, M AU - Arakawa, F AU - Kuwahara, M AU - Ozaki, H AU - Matsuoka, Y AU - Okazaki, M AU - Kanda, H PY - 1996/08// UR - http://view.ncbi.nlm.nih.gov/pubmed/8940726 ER -