A green tea polyphenol, epigalocatechin-3-gallate, induces apoptosis of human hepatocellular carcinoma, possibly through inhibition of Bcl-2 family proteins. Export

@article{citeulike:694280, abstract = {BACKGROUND/AIMS: A major polyphenol of green tea, epigallocatechin-3-gallate (EGCG), has previously been shown to induce cell-cycle arrest and apoptosis in various cancers. However, little is known about its effects on hepatocellular carcinomas (HCCs). METHODS: Four HCC cell lines, HLE, HepG2, HuH-7 and PLC/PRF/5, were treated with EGCG or vehicle. Cell viability was assessed by trypan blue staining and WST-8 assay. Cell-cycle, apoptosis and apoptosis-related proteins in HLE cells were evaluated by flow cytometry and Western blotting. The effect of EGCG was also studied in vivo using a xenograft model. The effect of co-treatment with EGCG and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) was also assessed. RESULTS: EGCG inhibited the growth of all HCC cell lines at concentrations of 50-100mug/ml. In HLE cells, EGCG induced apoptosis but not cell-cycle arrest and appears to have down-regulated Bcl-2alpha and Bcl-xl by inactivation of NF-kappaB. Oral administration of EGCG showed similar effects in HLE xenograft tumors. Co-treatment with EGCG and TRAIL synergistically induced apoptosis in HLE cells. CONCLUSIONS: EGCG induced apoptosis in HLE cells, both in vitro and in vivo. Moreover, it enhanced TRAIL-induced apoptosis. Therefore, EGCG treatment may be useful for improving the prognosis of HCCs.}, address = {Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine Graduate School of Medical Science, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan.}, author = {Nishikawa, T. and Nakajima, T. and Moriguchi, M. and Jo, M. and Sekoguchi, S. and Ishii, M. and Takashima, H. and Katagishi, T. and Kimura, H. and Minami, M. and Itoh, Y. and Kagawa, K. and Okanoue, T.}, citeulike-article-id = {694280}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.jhep.2005.11.045}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16481065}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16481065}, doi = {10.1016/j.jhep.2005.11.045}, issn = {0168-8278}, journal = {J Hepatol}, keywords = {anticancer, epigallocatechin, polyphenol}, month = {June}, number = {6}, pages = {1074--1082}, posted-at = {2006-06-13 04:47:18}, priority = {0}, title = {A green tea polyphenol, epigalocatechin-3-gallate, induces apoptosis of human hepatocellular carcinoma, possibly through inhibition of Bcl-2 family proteins.}, url = {http://dx.doi.org/10.1016/j.jhep.2005.11.045}, volume = {44}, year = {2006} }

TY - JOUR ID - citeulike:694280 L3 - citeulike-article-id:694280 N2 - BACKGROUND/AIMS: A major polyphenol of green tea, epigallocatechin-3-gallate (EGCG), has previously been shown to induce cell-cycle arrest and apoptosis in various cancers. However, little is known about its effects on hepatocellular carcinomas (HCCs). METHODS: Four HCC cell lines, HLE, HepG2, HuH-7 and PLC/PRF/5, were treated with EGCG or vehicle. Cell viability was assessed by trypan blue staining and WST-8 assay. Cell-cycle, apoptosis and apoptosis-related proteins in HLE cells were evaluated by flow cytometry and Western blotting. The effect of EGCG was also studied in vivo using a xenograft model. The effect of co-treatment with EGCG and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) was also assessed. RESULTS: EGCG inhibited the growth of all HCC cell lines at concentrations of 50-100mug/ml. In HLE cells, EGCG induced apoptosis but not cell-cycle arrest and appears to have down-regulated Bcl-2alpha and Bcl-xl by inactivation of NF-kappaB. Oral administration of EGCG showed similar effects in HLE xenograft tumors. Co-treatment with EGCG and TRAIL synergistically induced apoptosis in HLE cells. CONCLUSIONS: EGCG induced apoptosis in HLE cells, both in vitro and in vivo. Moreover, it enhanced TRAIL-induced apoptosis. Therefore, EGCG treatment may be useful for improving the prognosis of HCCs. IS - 6 JF - J Hepatol SN - 0168-8278 AD - Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine Graduate School of Medical Science, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan. EP - 1082 TI - A green tea polyphenol, epigalocatechin-3-gallate, induces apoptosis of human hepatocellular carcinoma, possibly through inhibition of Bcl-2 family proteins. VL - 44 SP - 1074 KW - anticancer KW - epigallocatechin KW - polyphenol AU - Nishikawa, T AU - Nakajima, T AU - Moriguchi, M AU - Jo, M AU - Sekoguchi, S AU - Ishii, M AU - Takashima, H AU - Katagishi, T AU - Kimura, H AU - Minami, M AU - Itoh, Y AU - Kagawa, K AU - Okanoue, T PY - 2006/06// UR - http://dx.doi.org/10.1016/j.jhep.2005.11.045 ER -