A modular microfluidic architecture for integrated biochemical analysis. Export

@article{citeulike:1465135, abstract = {Microfluidic laboratory-on-a-chip (LOC) systems based on a modular architecture are presented. The architecture is conceptualized on two levels: a single-chip level and a multiple-chip module (MCM) system level. At the individual chip level, a multilayer approach segregates components belonging to two fundamental categories: passive fluidic components (channels and reaction chambers) and active electromechanical control structures (sensors and actuators). This distinction is explicitly made to simplify the development process and minimize cost. Components belonging to these two categories are built separately on different physical layers and can communicate fluidically via cross-layer interconnects. The chip that hosts the electromechanical control structures is called the microfluidic breadboard (FBB). A single LOC module is constructed by attaching a chip comprised of a custom arrangement of fluid routing channels and reactors (passive chip) to the FBB. Many different LOC functions can be achieved by using different passive chips on an FBB with a standard resource configuration. Multiple modules can be interconnected to form a larger LOC system (MCM level). We demonstrated the utility of this architecture by developing systems for two separate biochemical applications: one for detection of protein markers of cancer and another for detection of metal ions. In the first case, free prostate-specific antigen was detected at 500 aM concentration by using a nanoparticle-based bio-bar-code protocol on a parallel MCM system. In the second case, we used a DNAzyme-based biosensor to identify the presence of Pb(2+) (lead) at a sensitivity of 500 nM in <1 nl of solution.}, address = {Department of Electrical and Computer Engineering, Micro and Nanotechnology Laboratory, and Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.}, author = {Shaikh, K. A. and Ryu, K. S. and Goluch, E. D. and Nam, J. M. and Liu, J. and Thaxton, C. S. and Chiesl, T. N. and Barron, A. E. and Lu, Y. and Mirkin, C. A. and Liu, C.}, citeulike-article-id = {1465135}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0504082102}, citeulike-linkout-1 = {http://www.pnas.org/cgi/content/abstract/102/28/9745}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/15985549}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=15985549}, day = {12}, doi = {10.1073/pnas.0504082102}, issn = {0027-8424}, journal = {Proc Natl Acad Sci U S A}, keywords = {bio-barcode, microfluidic-mixers, microfluidics}, month = {July}, number = {28}, pages = {9745--9750}, posted-at = {2008-03-21 18:39:23}, priority = {0}, title = {A modular microfluidic architecture for integrated biochemical analysis.}, url = {http://dx.doi.org/10.1073/pnas.0504082102}, volume = {102}, year = {2005} }

TY - JOUR ID - citeulike:1465135 L3 - citeulike-article-id:1465135 N2 - Microfluidic laboratory-on-a-chip (LOC) systems based on a modular architecture are presented. The architecture is conceptualized on two levels: a single-chip level and a multiple-chip module (MCM) system level. At the individual chip level, a multilayer approach segregates components belonging to two fundamental categories: passive fluidic components (channels and reaction chambers) and active electromechanical control structures (sensors and actuators). This distinction is explicitly made to simplify the development process and minimize cost. Components belonging to these two categories are built separately on different physical layers and can communicate fluidically via cross-layer interconnects. The chip that hosts the electromechanical control structures is called the microfluidic breadboard (FBB). A single LOC module is constructed by attaching a chip comprised of a custom arrangement of fluid routing channels and reactors (passive chip) to the FBB. Many different LOC functions can be achieved by using different passive chips on an FBB with a standard resource configuration. Multiple modules can be interconnected to form a larger LOC system (MCM level). We demonstrated the utility of this architecture by developing systems for two separate biochemical applications: one for detection of protein markers of cancer and another for detection of metal ions. In the first case, free prostate-specific antigen was detected at 500 aM concentration by using a nanoparticle-based bio-bar-code protocol on a parallel MCM system. In the second case, we used a DNAzyme-based biosensor to identify the presence of Pb(2+) (lead) at a sensitivity of 500 nM in <1 nl of solution. IS - 28 JF - Proc Natl Acad Sci U S A SN - 0027-8424 AD - Department of Electrical and Computer Engineering, Micro and Nanotechnology Laboratory, and Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. EP - 9750 TI - A modular microfluidic architecture for integrated biochemical analysis. VL - 102 SP - 9745 KW - bio-barcode KW - microfluidic-mixers KW - microfluidics AU - Shaikh, KA AU - Ryu, KS AU - Goluch, ED AU - Nam, JM AU - Liu, J AU - Thaxton, CS AU - Chiesl, TN AU - Barron, AE AU - Lu, Y AU - Mirkin, CA AU - Liu, C PY - 2005/07/12/ UR - http://dx.doi.org/10.1073/pnas.0504082102 ER -