Microfabricated bioprocessor for integrated nanoliter-scale Sanger DNA sequencing. Export

@article{citeulike:1641276, abstract = {An efficient, nanoliter-scale microfabricated bioprocessor integrating all three Sanger sequencing steps, thermal cycling, sample purification, and capillary electrophoresis, has been developed and evaluated. Hybrid glass-polydimethylsiloxane (PDMS) wafer-scale construction is used to combine 250-nl reactors, affinity-capture purification chambers, high-performance capillary electrophoresis channels, and pneumatic valves and pumps onto a single microfabricated device. Lab-on-a-chip-level integration enables complete Sanger sequencing from only 1 fmol of DNA template. Up to 556 continuous bases were sequenced with 99\% accuracy, demonstrating read lengths required for de novo sequencing of human and other complex genomes. The performance of this miniaturized DNA sequencer provides a benchmark for predicting the ultimate cost and efficiency limits of Sanger sequencing.}, address = {University of California, San Francisco/University of California, Berkeley, Joint Bioengineering Graduate Group, Berkeley, CA 94720, USA.}, author = {Blazej, R. G. and Kumaresan, P. and Mathies, R. A.}, citeulike-article-id = {1641276}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0602476103}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16648246}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16648246}, citeulike-linkout-3 = {http://www.scopus.com/record/display.url?view=extended&origin=resultslist&eid=2-s2.0-33646555429}, day = {9}, doi = {10.1073/pnas.0602476103}, issn = {0027-8424}, journal = {Proc Natl Acad Sci U S A}, keywords = {capillary-electrophoresis, dna, microfluidics, sequencing}, month = {May}, number = {19}, pages = {7240--7245}, posted-at = {2008-04-08 16:32:09}, priority = {2}, title = {Microfabricated bioprocessor for integrated nanoliter-scale Sanger DNA sequencing.}, url = {http://dx.doi.org/10.1073/pnas.0602476103}, volume = {103}, year = {2006} }

TY - JOUR ID - citeulike:1641276 L3 - citeulike-article-id:1641276 N2 - An efficient, nanoliter-scale microfabricated bioprocessor integrating all three Sanger sequencing steps, thermal cycling, sample purification, and capillary electrophoresis, has been developed and evaluated. Hybrid glass-polydimethylsiloxane (PDMS) wafer-scale construction is used to combine 250-nl reactors, affinity-capture purification chambers, high-performance capillary electrophoresis channels, and pneumatic valves and pumps onto a single microfabricated device. Lab-on-a-chip-level integration enables complete Sanger sequencing from only 1 fmol of DNA template. Up to 556 continuous bases were sequenced with 99% accuracy, demonstrating read lengths required for de novo sequencing of human and other complex genomes. The performance of this miniaturized DNA sequencer provides a benchmark for predicting the ultimate cost and efficiency limits of Sanger sequencing. IS - 19 JF - Proc Natl Acad Sci U S A SN - 0027-8424 AD - University of California, San Francisco/University of California, Berkeley, Joint Bioengineering Graduate Group, Berkeley, CA 94720, USA. EP - 7245 TI - Microfabricated bioprocessor for integrated nanoliter-scale Sanger DNA sequencing. VL - 103 SP - 7240 KW - capillary-electrophoresis KW - dna KW - microfluidics KW - sequencing AU - Blazej, RG AU - Kumaresan, P AU - Mathies, RA PY - 2006/05/09/ UR - http://dx.doi.org/10.1073/pnas.0602476103 ER -