Self-Contained, Fully Integrated Biochip for Sample Preparation, Polymerase Chain Reaction Amplification, and DNA Microarray Detection Export

@article{citeulike:2638022, abstract = {A fully integrated biochip device that consists of microfluidic mixers, valves, pumps, channels, chambers, heaters, and DNA microarray sensors was developed to perform DNA analysis of complex biological sample solutions. Sample preparation (including magnetic bead-based cell capture, cell preconcentration and purification, and cell lysis), polymerase chain reaction, DNA hybridization, and electrochemical detection were performed in this fully automated and miniature device. Cavitation microstreaming was implemented to enhance target cell capture from whole blood samples using immunomagnetic beads and accelerate DNA hybridization reaction. Thermally actuated paraffin-based microvalves were developed to regulate flows. Electrochemical pumps and thermopneumatic pumps were integrated on the chip to provide pumping of liquid solutions. The device is completely self-contained: no external pressure sources, fluid storage, mechanical pumps, or valves are necessary for fluid manipulation, thus eliminating possible sample contamination and simplifying device operation. Pathogenic bacteria detection from approximately milliliters of whole blood samples and single-nucleotide polymorphism analysis directly from diluted blood were demonstrated. The device provides a cost-effective solution to direct sample-to-answer genetic analysis and thus has a potential impact in the fields of point-of-care genetic analysis, environmental testing, and biological warfare agent detection.}, address = {Los Alamos National Laboratory, Los Alamos, NM 87545, United States}, author = {Liu, R. H. and Yang, J. and Lenigk, R. and Bonanno, J. and Grodzinski, P.}, citeulike-article-id = {2638022}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/ac0353029}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/ac0353029}, citeulike-linkout-2 = {http://www.scopus.com/record/display.url?view=extended&origin=resultslist&eid=2-s2.0-1842477059}, doi = {10.1021/ac0353029}, journal = {Anal. Chem.}, keywords = {blood, dna, microfluidic-mixers, microfluidics, pressure-driven-flow, sample-prep}, number = {7}, pages = {1824--1831}, posted-at = {2008-04-07 16:27:18}, priority = {2}, title = {Self-Contained, Fully Integrated Biochip for Sample Preparation, Polymerase Chain Reaction Amplification, and DNA Microarray Detection}, url = {http://dx.doi.org/10.1021/ac0353029}, volume = {76}, year = {2004} }

TY - JOUR ID - citeulike:2638022 L3 - citeulike-article-id:2638022 N2 - A fully integrated biochip device that consists of microfluidic mixers, valves, pumps, channels, chambers, heaters, and DNA microarray sensors was developed to perform DNA analysis of complex biological sample solutions. Sample preparation (including magnetic bead-based cell capture, cell preconcentration and purification, and cell lysis), polymerase chain reaction, DNA hybridization, and electrochemical detection were performed in this fully automated and miniature device. Cavitation microstreaming was implemented to enhance target cell capture from whole blood samples using immunomagnetic beads and accelerate DNA hybridization reaction. Thermally actuated paraffin-based microvalves were developed to regulate flows. Electrochemical pumps and thermopneumatic pumps were integrated on the chip to provide pumping of liquid solutions. The device is completely self-contained: no external pressure sources, fluid storage, mechanical pumps, or valves are necessary for fluid manipulation, thus eliminating possible sample contamination and simplifying device operation. Pathogenic bacteria detection from approximately milliliters of whole blood samples and single-nucleotide polymorphism analysis directly from diluted blood were demonstrated. The device provides a cost-effective solution to direct sample-to-answer genetic analysis and thus has a potential impact in the fields of point-of-care genetic analysis, environmental testing, and biological warfare agent detection. IS - 7 JF - Anal. Chem. AD - Los Alamos National Laboratory, Los Alamos, NM 87545, United States EP - 1831 TI - Self-Contained, Fully Integrated Biochip for Sample Preparation, Polymerase Chain Reaction Amplification, and DNA Microarray Detection VL - 76 SP - 1824 KW - blood KW - dna KW - microfluidic-mixers KW - microfluidics KW - pressure-driven-flow KW - sample-prep AU - Liu, RH AU - Yang, J AU - Lenigk, R AU - Bonanno, J AU - Grodzinski, P PY - 2004/// UR - http://dx.doi.org/10.1021/ac0353029 ER -