Microfluidic sonicator for real-time disruption of eukaryotic cells and bacterial spores for DNA analysis Export

@article{citeulike:2642984, abstract = {Biologic agent screening is a three-step process: lysis of host cell membranes or walls to release their DNA, polymerase chain reaction to amplify the genetic material and screening for distinguishing genetic signatures. Macrofluidic devices commonly use sonication as a lysis method. Here, we present a piezoelectric microfluidic minisonicator and test its performance. Eukaryotic human leukemia HL-60 cells and Bacillus subtilis bacterial spores were lysed as they passed through a microfluidic channel at 50 ?L/min and 5 ?L/min, respectively, in the absence of any chemical denaturants, enzymes or microparticles. We used fluorescence-activated cell sorting and hematocytometry to measure 80\% lysis of HL-60 cells after 3 s of sonication. Real-time polymerase chain reaction indicated 50\% lysis of B. subtilis spores with 30 s of sonication. Advantages of the minisonicator over macrofluidic implementations include a small sample volume (2.5 ?L), reduced energy consumption and compatibility with other microfluidic blocks. These features make this device an attractive option for "lab-on-a-chip" and portable applications. {\copyright} 2005 World Federation for Ultrasound in Medicine \& Biology.}, address = {Box 239 Vanderbilt, Harvard Medical School, 107 Avenue Louis Pasteur, Boston, MA 02115, United States}, author = {Marentis, T. C. and Kusler, B. and Yaralioglu, G. G. and Liu, S. and H{\ae}ggstro?m, E. O. and Khuri-Yakub, B. T.}, citeulike-article-id = {2642984}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.ultrasmedbio.2005.05.005}, citeulike-linkout-1 = {http://www.scopus.com/record/display.url?view=extended&origin=resultslist&eid=2-s2.0-24944538785}, doi = {10.1016/j.ultrasmedbio.2005.05.005}, journal = {Ultrasound Med. Biol.}, keywords = {lysis, microfluidics, sample-prep}, number = {9}, pages = {1265--1277}, posted-at = {2008-04-08 22:51:38}, priority = {2}, title = {Microfluidic sonicator for real-time disruption of eukaryotic cells and bacterial spores for DNA analysis}, url = {http://dx.doi.org/10.1016/j.ultrasmedbio.2005.05.005}, volume = {31}, year = {2005} }

TY - JOUR ID - citeulike:2642984 L3 - citeulike-article-id:2642984 N2 - Biologic agent screening is a three-step process: lysis of host cell membranes or walls to release their DNA, polymerase chain reaction to amplify the genetic material and screening for distinguishing genetic signatures. Macrofluidic devices commonly use sonication as a lysis method. Here, we present a piezoelectric microfluidic minisonicator and test its performance. Eukaryotic human leukemia HL-60 cells and Bacillus subtilis bacterial spores were lysed as they passed through a microfluidic channel at 50 ?L/min and 5 ?L/min, respectively, in the absence of any chemical denaturants, enzymes or microparticles. We used fluorescence-activated cell sorting and hematocytometry to measure 80% lysis of HL-60 cells after 3 s of sonication. Real-time polymerase chain reaction indicated 50% lysis of B. subtilis spores with 30 s of sonication. Advantages of the minisonicator over macrofluidic implementations include a small sample volume (2.5 ?L), reduced energy consumption and compatibility with other microfluidic blocks. These features make this device an attractive option for "lab-on-a-chip" and portable applications. © 2005 World Federation for Ultrasound in Medicine & Biology. IS - 9 JF - Ultrasound Med. Biol. AD - Box 239 Vanderbilt, Harvard Medical School, 107 Avenue Louis Pasteur, Boston, MA 02115, United States EP - 1277 TI - Microfluidic sonicator for real-time disruption of eukaryotic cells and bacterial spores for DNA analysis VL - 31 SP - 1265 KW - lysis KW - microfluidics KW - sample-prep AU - Marentis, TC AU - Kusler, B AU - Yaralioglu, GG AU - Liu, S AU - Hæggstro?m, EO AU - Khuri-Yakub, BT PY - 2005/// UR - http://dx.doi.org/10.1016/j.ultrasmedbio.2005.05.005 ER -