Microchip Device for Cell Lysis, Multiplex PCR Amplification, and Electrophoretic Sizing Export

@article{citeulike:2647131, abstract = {The steps of cell lysis, multiplex PCR amplification, and electrophoretic analysis are executed sequentially on a monolithic microchip device. The entire microchip is thermally cycled to lyse cells and to amplify DNA, and the products are then analyzed using a sieving medium for size separation and an intercalating dye for fluorescence detection. Using a standard PCR protocol, a 500-base pair (bp) region of bacteriophage ? DNA and 154-, 264-, 346-, 410-, and 550-bp regions of E. coli genomic and plasmid DNAs are amplified. The electrophoretic analysis of the products is executed in <3 min following amplification using hydroxyethyl cellulose or poly(dimethylacrylamide) sieving gels. Product sizing is demonstrated by proportioning the amplified product with a DNA sizing ladder.}, address = {Oak Ridge National Laboratory, P.O. Box 2008, Oak Ridge, TN 37831-6142, United States}, author = {Waters, L. C. and Jacobson, S. C. and Kroutchinina, N. and Khandurina, J. and Foote, R. S. and Ramsey, J. M.}, citeulike-article-id = {2647131}, citeulike-linkout-0 = {http://www.scopus.com/record/display.url?view=extended&origin=resultslist&eid=2-s2.0-0031607381}, journal = {Anal. Chem.}, keywords = {capillary-electrophoresis, dna, lysis, microfluidics, pcr, sample-prep}, number = {1}, pages = {158--162}, posted-at = {2008-04-09 20:29:51}, priority = {2}, title = {Microchip Device for Cell Lysis, Multiplex PCR Amplification, and Electrophoretic Sizing}, url = {http://www.scopus.com/record/display.url?view=extended&origin=resultslist&eid=2-s2.0-0031607381}, volume = {70}, year = {1998} }

TY - JOUR ID - citeulike:2647131 L3 - citeulike-article-id:2647131 N2 - The steps of cell lysis, multiplex PCR amplification, and electrophoretic analysis are executed sequentially on a monolithic microchip device. The entire microchip is thermally cycled to lyse cells and to amplify DNA, and the products are then analyzed using a sieving medium for size separation and an intercalating dye for fluorescence detection. Using a standard PCR protocol, a 500-base pair (bp) region of bacteriophage ? DNA and 154-, 264-, 346-, 410-, and 550-bp regions of E. coli genomic and plasmid DNAs are amplified. The electrophoretic analysis of the products is executed in <3 min following amplification using hydroxyethyl cellulose or poly(dimethylacrylamide) sieving gels. Product sizing is demonstrated by proportioning the amplified product with a DNA sizing ladder. IS - 1 JF - Anal. Chem. AD - Oak Ridge National Laboratory, P.O. Box 2008, Oak Ridge, TN 37831-6142, United States EP - 162 TI - Microchip Device for Cell Lysis, Multiplex PCR Amplification, and Electrophoretic Sizing VL - 70 SP - 158 KW - capillary-electrophoresis KW - dna KW - lysis KW - microfluidics KW - pcr KW - sample-prep AU - Waters, LC AU - Jacobson, SC AU - Kroutchinina, N AU - Khandurina, J AU - Foote, RS AU - Ramsey, JM PY - 1998/// UR - http://www.scopus.com/record/display.url?view=extended&origin=resultslist&eid=2-s2.0-0031607381 ER -