Identification of an atypical integron carrying an IS26-disrupted aadA1 gene cassette in Acinetobacter baumannii. Export

@article{sul1-is26-inti1-2008, abstract = {An unusual class 1 integron was identified that carries an IS26-disrupted aadA1 gene cassette (designated as 'integron-IS26') in an imipenem-resistant Acinetobacter baumannii (IRAB) outbreak strain. DNA sequencing revealed that integron-IS26 contained two gene cassettes, the aac(6')-Im cassette and a peculiar aadA1 cassette that was disrupted by IS26 (disrupted aadA1 cassette). Southern blotting localised integron-IS26 to the chromosome. Nested polymerase chain reaction (PCR) was performed to define the frequency of integron-IS26 in five groups of bacteria. Nested PCR identified integron-IS26 in 19 (73.1\%) of 26 clinical outbreak strains of IRAB, 10 (100\%) of 10 IRAB isolated from environmental cultures, 3 (13.0\%) of 23 imipenem-susceptible A. baumannii (ISAB) non-outbreak strains, 1 (3.6\%) of 28 netilmicin- and tobramycin-resistant A. baumannii and none of the netilmicin- and tobramycin-resistant Pseudomonas aeruginosa. In conclusion, we have identified a novel class I integron that carries the aac(6')-Im cassette and an IS26-disrupted copy of aadA1 (integron-IS26) in most IRAB outbreak strains and in a few ISAB non-outbreak control strains. Integron-IS26 is located chromosomally.}, author = {Han, H. L. and Jang, S. J. and Park, G. and Kook, J. K. and Shin, J. H. and Shin, S. H. and Kim, D. M. and Cheon, J. S. and Moon, D. S. and Park, Y. J.}, citeulike-article-id = {4919991}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.ijantimicag.2008.03.009}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18565738}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18565738}, doi = {10.1016/j.ijantimicag.2008.03.009}, issn = {0924-8579}, journal = {International journal of antimicrobial agents}, keywords = {integron, inti1, is26, sul1}, month = {August}, number = {2}, pages = {165--169}, posted-at = {2009-06-21 13:40:12}, priority = {2}, title = {Identification of an atypical integron carrying an IS26-disrupted aadA1 gene cassette in Acinetobacter baumannii.}, url = {http://dx.doi.org/10.1016/j.ijantimicag.2008.03.009}, volume = {32}, year = {2008} }

TY - JOUR ID - sul1-is26-inti1-2008 L3 - citeulike-article-id:4919991 N2 - An unusual class 1 integron was identified that carries an IS26-disrupted aadA1 gene cassette (designated as 'integron-IS26') in an imipenem-resistant Acinetobacter baumannii (IRAB) outbreak strain. DNA sequencing revealed that integron-IS26 contained two gene cassettes, the aac(6')-Im cassette and a peculiar aadA1 cassette that was disrupted by IS26 (disrupted aadA1 cassette). Southern blotting localised integron-IS26 to the chromosome. Nested polymerase chain reaction (PCR) was performed to define the frequency of integron-IS26 in five groups of bacteria. Nested PCR identified integron-IS26 in 19 (73.1%) of 26 clinical outbreak strains of IRAB, 10 (100%) of 10 IRAB isolated from environmental cultures, 3 (13.0%) of 23 imipenem-susceptible A. baumannii (ISAB) non-outbreak strains, 1 (3.6%) of 28 netilmicin- and tobramycin-resistant A. baumannii and none of the netilmicin- and tobramycin-resistant Pseudomonas aeruginosa. In conclusion, we have identified a novel class I integron that carries the aac(6')-Im cassette and an IS26-disrupted copy of aadA1 (integron-IS26) in most IRAB outbreak strains and in a few ISAB non-outbreak control strains. Integron-IS26 is located chromosomally. IS - 2 JF - International journal of antimicrobial agents SN - 0924-8579 EP - 169 TI - Identification of an atypical integron carrying an IS26-disrupted aadA1 gene cassette in Acinetobacter baumannii. VL - 32 SP - 165 KW - integron KW - inti1 KW - is26 KW - sul1 AU - Han, HL AU - Jang, SJ AU - Park, G AU - Kook, JK AU - Shin, JH AU - Shin, SH AU - Kim, DM AU - Cheon, JS AU - Moon, DS AU - Park, YJ PY - 2008/08// UR - http://dx.doi.org/10.1016/j.ijantimicag.2008.03.009 ER -