Complete nucleotide sequence and molecular characterization of ViaB region encoding Vi antigen in Salmonella typhi. Export

@article{viaB-seq-1993, abstract = {Plasmid pGBM124, which contains a 14-kb Salmonella typhi chromosomal DNA fragment capable of producing the Vi antigen in Escherichia coli HB101 and ViaB-deleted S. typhi GIFU 10007-3, was studied. We determined the complete nucleotide sequence of this fragment and found 11 open reading frames. Mutagenesis, subcloning, and complementation analysis showed that three genes (vipA, vipB, and vipC) are involved in biosynthesis of the Vi polysaccharide. The putative primary amino acid sequence suggests that both vipA and vipB encode the NAD- or NADP-dependent enzymes to synthesize the nucleotide sugar for the Vi polysaccharide. Five genes (vexA, vexB, vexC, vexD, and vexE) may be involved in translocation of the Vi polysaccharide. Proteins VexA, VexB, VexC, and VexD had moderate similarities to components of group II capsule transporters, and the VexC protein had a putative ATP-binding site. These data indicate that the transport system for the Vi polysaccharide belongs to the ATP-binding cassette transporters. By using the isogenic Vi+ and Vi- strains constructed in this study, we reconfirmed that the Vi antigen is necessary for the serum resistance of S. typhi.}, author = {Hashimoto, Y. and Li, N. and Yokoyama, H. and Ezaki, T.}, citeulike-article-id = {5179208}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/8331073}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=8331073}, issn = {0021-9193}, journal = {Journal of bacteriology}, keywords = {locus, sequence, viab}, month = {July}, number = {14}, pages = {4456--4465}, posted-at = {2009-07-16 14:09:10}, priority = {2}, title = {Complete nucleotide sequence and molecular characterization of ViaB region encoding Vi antigen in Salmonella typhi.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/8331073}, volume = {175}, year = {1993} }

TY - JOUR ID - viaB-seq-1993 L3 - citeulike-article-id:5179208 N2 - Plasmid pGBM124, which contains a 14-kb Salmonella typhi chromosomal DNA fragment capable of producing the Vi antigen in Escherichia coli HB101 and ViaB-deleted S. typhi GIFU 10007-3, was studied. We determined the complete nucleotide sequence of this fragment and found 11 open reading frames. Mutagenesis, subcloning, and complementation analysis showed that three genes (vipA, vipB, and vipC) are involved in biosynthesis of the Vi polysaccharide. The putative primary amino acid sequence suggests that both vipA and vipB encode the NAD- or NADP-dependent enzymes to synthesize the nucleotide sugar for the Vi polysaccharide. Five genes (vexA, vexB, vexC, vexD, and vexE) may be involved in translocation of the Vi polysaccharide. Proteins VexA, VexB, VexC, and VexD had moderate similarities to components of group II capsule transporters, and the VexC protein had a putative ATP-binding site. These data indicate that the transport system for the Vi polysaccharide belongs to the ATP-binding cassette transporters. By using the isogenic Vi+ and Vi- strains constructed in this study, we reconfirmed that the Vi antigen is necessary for the serum resistance of S. typhi. IS - 14 JF - Journal of bacteriology SN - 0021-9193 EP - 4465 TI - Complete nucleotide sequence and molecular characterization of ViaB region encoding Vi antigen in Salmonella typhi. VL - 175 SP - 4456 KW - locus KW - sequence KW - viab AU - Hashimoto, Y AU - Li, N AU - Yokoyama, H AU - Ezaki, T PY - 1993/07// UR - http://view.ncbi.nlm.nih.gov/pubmed/8331073 ER -