Antioxidant defense response in a galling insect Export

@article{citeulike:4270236, abstract = {10.1073/pnas.0604722104 Herbivorous insect species are constantly challenged with reactive oxygen species (ROS) generated from endogenous and exogenous sources. ROS produced within insects because of stress and prooxidant allelochemicals produced by host plants in response to herbivory require a complex mode of antioxidant defense during insect/plant interactions. Some insect herbivores have a midgut-based defense against the suite of ROS encountered. Because the Hessian fly () is the major insect pest of wheat worldwide, and an emerging model for all gall midges, we investigated its antioxidant responses during interaction with its host plant. Quantitative data for two phospholipid glutathione peroxidases ( and ), two catalases ( and ), and two superoxide dismutases ( and ) revealed high levels of all of the mRNAs in the midgut of larvae on susceptible wheat (compatible interaction). During development of the Hessian fly on susceptible wheat, a differential expression pattern was observed for all six genes. Analysis of larvae on resistant wheat (incompatible interaction) compared with larvae on susceptible wheat showed increased levels of mRNAs in larvae on resistant wheat for all of the antioxidant genes except and . We postulate that the increased mRNA levels of , , , and reflect responses to ROS encountered by larvae while feeding on resistant wheat seedlings and/or ROS generated endogenously in larvae because of stress/starvation. These results provide an opportunity to understand the cooperative antioxidant defense responses in the Hessian fly/wheat interaction and may be applicable to other insect/plant interactions.}, author = {Mittapalli, Omprakash and Neal, Jonathan J. and Shukle, Richard H.}, citeulike-article-id = {4270236}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0604722104}, citeulike-linkout-1 = {http://www.pnas.org/content/104/6/1889.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/104/6/1889.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/17261812}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=17261812}, day = {6}, doi = {10.1073/pnas.0604722104}, journal = {Proceedings of the National Academy of Sciences}, keywords = {antioxidant, defence, gall, insect}, month = {February}, number = {6}, pages = {1889--1894}, posted-at = {2009-04-03 17:25:38}, priority = {2}, title = {Antioxidant defense response in a galling insect}, url = {http://dx.doi.org/10.1073/pnas.0604722104}, volume = {104}, year = {2007} }

TY - JOUR ID - citeulike:4270236 L3 - citeulike-article-id:4270236 N2 - 10.1073/pnas.0604722104 Herbivorous insect species are constantly challenged with reactive oxygen species (ROS) generated from endogenous and exogenous sources. ROS produced within insects because of stress and prooxidant allelochemicals produced by host plants in response to herbivory require a complex mode of antioxidant defense during insect/plant interactions. Some insect herbivores have a midgut-based defense against the suite of ROS encountered. Because the Hessian fly () is the major insect pest of wheat worldwide, and an emerging model for all gall midges, we investigated its antioxidant responses during interaction with its host plant. Quantitative data for two phospholipid glutathione peroxidases ( and ), two catalases ( and ), and two superoxide dismutases ( and ) revealed high levels of all of the mRNAs in the midgut of larvae on susceptible wheat (compatible interaction). During development of the Hessian fly on susceptible wheat, a differential expression pattern was observed for all six genes. Analysis of larvae on resistant wheat (incompatible interaction) compared with larvae on susceptible wheat showed increased levels of mRNAs in larvae on resistant wheat for all of the antioxidant genes except and . We postulate that the increased mRNA levels of , , , and reflect responses to ROS encountered by larvae while feeding on resistant wheat seedlings and/or ROS generated endogenously in larvae because of stress/starvation. These results provide an opportunity to understand the cooperative antioxidant defense responses in the Hessian fly/wheat interaction and may be applicable to other insect/plant interactions. IS - 6 JF - Proceedings of the National Academy of Sciences EP - 1894 TI - Antioxidant defense response in a galling insect VL - 104 SP - 1889 KW - antioxidant KW - defence KW - gall KW - insect AU - Mittapalli, Omprakash AU - Neal, Jonathan AU - Shukle, Richard PY - 2007/02/06/ UR - http://dx.doi.org/10.1073/pnas.0604722104 ER -