Gene expression and metabolite profiling of Populus euphratica growing in the Negev desert. Export

@article{citeulike:441749, abstract = {ABSTRACT : BACKGROUND : Plants growing in their natural habitat represent a valuable resource for elucidating mechanisms of acclimation to environmental constraints. Populus euphratica is a salt-tolerant tree species growing in saline semi-arid areas. To identify genes involved in abiotic stress responses under natural conditions we constructed several normalized and subtracted cDNA libraries from control, stress-exposed and desert-grown P. euphratica trees. In addition, we identified several metabolites in desert-grown P. euphratica trees. RESULTS : About 14,000 expressed sequence tag (EST) sequences were obtained with a good representation of genes putatively involved in resistance and tolerance to salt and other abiotic stresses. A P. euphratica DNA microarray with a uni-gene set of ESTs representing approximately 6,340 different genes was constructed. The microarray was used to study gene expression in adult P. euphratica trees growing in the desert canyon of Ein Avdat in Israel. In parallel, 22 selected metabolites were profiled in the same trees. CONCLUSION : Of the obtained ESTs, 98\% were found in the sequenced P. trichocarpa genome and 74\% in other Populus EST collections. This implies that the P. euphratica genome does not contain different genes per se, but that regulation of gene expression might be different and that P. euphratica expresses a different set of genes that contribute to adaptation to saline growth conditions. Also, all of the five measured amino acids show increased levels in trees growing in the more saline soil.}, address = {Plant Biology, Department of Biological and Environmental Sciences, University of Helsinki, P,O, Box 65, Viikinkaari 1, FIN-00014 Helsinki, Finland. Mikael.brosche@helsinki.fi.}, author = {Brosch\'{e}, M. and Vinocur, B. and Alatalo, E. R. and Lamminm\"{a}ki, A. and Teichmann, T. and Ottow, E. A. and Djilianov, D. and Afif, D. and Bogeat-Triboulot, M. B. and Altman, A. and Polle, A. and Dreyer, E. and Rudd, S. and Paulin, L. and Auvinen, P. and Kangasj\"{a}rvi, J.}, citeulike-article-id = {441749}, citeulike-linkout-0 = {http://dx.doi.org/10.1186/gb-2005-6-12-r101}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16356264}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16356264}, doi = {10.1186/gb-2005-6-12-r101}, issn = {1465-6914}, journal = {Genome Biol}, keywords = {expression, gene, metabolite, populus, profiling}, number = {12}, posted-at = {2009-06-11 15:15:43}, priority = {2}, title = {Gene expression and metabolite profiling of Populus euphratica growing in the Negev desert.}, url = {http://dx.doi.org/10.1186/gb-2005-6-12-r101}, volume = {6}, year = {2005} }

TY - JOUR ID - citeulike:441749 L3 - citeulike-article-id:441749 N2 - ABSTRACT : BACKGROUND : Plants growing in their natural habitat represent a valuable resource for elucidating mechanisms of acclimation to environmental constraints. Populus euphratica is a salt-tolerant tree species growing in saline semi-arid areas. To identify genes involved in abiotic stress responses under natural conditions we constructed several normalized and subtracted cDNA libraries from control, stress-exposed and desert-grown P. euphratica trees. In addition, we identified several metabolites in desert-grown P. euphratica trees. RESULTS : About 14,000 expressed sequence tag (EST) sequences were obtained with a good representation of genes putatively involved in resistance and tolerance to salt and other abiotic stresses. A P. euphratica DNA microarray with a uni-gene set of ESTs representing approximately 6,340 different genes was constructed. The microarray was used to study gene expression in adult P. euphratica trees growing in the desert canyon of Ein Avdat in Israel. In parallel, 22 selected metabolites were profiled in the same trees. CONCLUSION : Of the obtained ESTs, 98% were found in the sequenced P. trichocarpa genome and 74% in other Populus EST collections. This implies that the P. euphratica genome does not contain different genes per se, but that regulation of gene expression might be different and that P. euphratica expresses a different set of genes that contribute to adaptation to saline growth conditions. Also, all of the five measured amino acids show increased levels in trees growing in the more saline soil. IS - 12 JF - Genome Biol SN - 1465-6914 AD - Plant Biology, Department of Biological and Environmental Sciences, University of Helsinki, P,O, Box 65, Viikinkaari 1, FIN-00014 Helsinki, Finland. Mikael.brosche@helsinki.fi. TI - Gene expression and metabolite profiling of Populus euphratica growing in the Negev desert. VL - 6 KW - expression KW - gene KW - metabolite KW - populus KW - profiling AU - Brosché, M AU - Vinocur, B AU - Alatalo, ER AU - Lamminmäki, A AU - Teichmann, T AU - Ottow, EA AU - Djilianov, D AU - Afif, D AU - Bogeat-Triboulot, MB AU - Altman, A AU - Polle, A AU - Dreyer, E AU - Rudd, S AU - Paulin, L AU - Auvinen, P AU - Kangasjärvi, J PY - 2005/// UR - http://dx.doi.org/10.1186/gb-2005-6-12-r101 ER -