Salicyl Alcohol Oxidase of the Chemical Defense Secretion of Two Chrysomelid Leaf Beetles: MOLECULAR AND FUNCTIONAL CHARACTERIZATION OF TWO NEW MEMBERS OF THE GLUCOSE-METHANOL-CHOLINE OXIDOREDUCTASE GENE FAMILY Export

@article{citeulike:4518045, abstract = {Salicyl alcohol oxidase is an extracellular enzyme that occurs in glandular reservoirs of chrysomelid leaf beetle larvae and catalyzes the formation of salicylaldehyde, a volatile deterrent used by the larvae against predators. Salicyl alcohol is the hydrolysis product of salicin, a plant-derived precursor taken up by the beetle larvae from the leaves of willow and poplar trees. The cDNA encoding salicyl alcohol oxidase from two related species Chrysomela tremulae and Chrysomela populi has been identified, cloned, and expressed in an active form in Escherichia coli. The open reading frame of 623 amino acids begins in both enzymes with an N-terminal signal peptide of 21 amino acids. Sequence comparison has revealed that salicyl alcohol oxidase belongs to the family of glucose-methanol-choline oxidoreductase-like sequences with mostly unknown function. Enzymes of this family share similar overall structure with an essentially identical FAD-binding site but possess different catalytic activities. The data suggest that salicyl alcohol oxidase, essential for the activation of the plant-derived precursor salicin, was originally recruited from an oxidase involved in the autogenous biosynthesis of iridoid monoterpenes and found in related chrysomelid leaf beetle species. 10.1074/jbc.M802236200}, author = {Michalski, Carmen and Mohagheghi, Hoda and Nimtz, Manfred and Pasteels, Jacques and Ober, Dietrich}, citeulike-article-id = {4518045}, citeulike-linkout-0 = {http://dx.doi.org/10.1074/jbc.M802236200}, citeulike-linkout-1 = {http://www.jbc.org/cgi/content/abstract/283/28/19219}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18482980}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18482980}, day = {11}, doi = {10.1074/jbc.M802236200}, journal = {J. Biol. Chem.}, keywords = {beetle, chemical, chrysomelid, defence, leaf, molecular}, month = {July}, number = {28}, pages = {19219--19228}, posted-at = {2009-05-14 13:41:00}, priority = {2}, title = {Salicyl Alcohol Oxidase of the Chemical Defense Secretion of Two Chrysomelid Leaf Beetles: MOLECULAR AND FUNCTIONAL CHARACTERIZATION OF TWO NEW MEMBERS OF THE GLUCOSE-METHANOL-CHOLINE OXIDOREDUCTASE GENE FAMILY}, url = {http://dx.doi.org/10.1074/jbc.M802236200}, volume = {283}, year = {2008} }

TY - JOUR ID - citeulike:4518045 L3 - citeulike-article-id:4518045 N2 - Salicyl alcohol oxidase is an extracellular enzyme that occurs in glandular reservoirs of chrysomelid leaf beetle larvae and catalyzes the formation of salicylaldehyde, a volatile deterrent used by the larvae against predators. Salicyl alcohol is the hydrolysis product of salicin, a plant-derived precursor taken up by the beetle larvae from the leaves of willow and poplar trees. The cDNA encoding salicyl alcohol oxidase from two related species Chrysomela tremulae and Chrysomela populi has been identified, cloned, and expressed in an active form in Escherichia coli. The open reading frame of 623 amino acids begins in both enzymes with an N-terminal signal peptide of 21 amino acids. Sequence comparison has revealed that salicyl alcohol oxidase belongs to the family of glucose-methanol-choline oxidoreductase-like sequences with mostly unknown function. Enzymes of this family share similar overall structure with an essentially identical FAD-binding site but possess different catalytic activities. The data suggest that salicyl alcohol oxidase, essential for the activation of the plant-derived precursor salicin, was originally recruited from an oxidase involved in the autogenous biosynthesis of iridoid monoterpenes and found in related chrysomelid leaf beetle species. 10.1074/jbc.M802236200 IS - 28 JF - J. Biol. Chem. EP - 19228 TI - Salicyl Alcohol Oxidase of the Chemical Defense Secretion of Two Chrysomelid Leaf Beetles: MOLECULAR AND FUNCTIONAL CHARACTERIZATION OF TWO NEW MEMBERS OF THE GLUCOSE-METHANOL-CHOLINE OXIDOREDUCTASE GENE FAMILY VL - 283 SP - 19219 KW - beetle KW - chemical KW - chrysomelid KW - defence KW - leaf KW - molecular AU - Michalski, Carmen AU - Mohagheghi, Hoda AU - Nimtz, Manfred AU - Pasteels, Jacques AU - Ober, Dietrich PY - 2008/07/11/ UR - http://dx.doi.org/10.1074/jbc.M802236200 ER -