Leukocyte analysis and differentiation using high speed microfluidic single cell impedance cytometry. Export

@article{citeulike:5730503, abstract = {Miniature high speed label-free cell analysis systems have yet to be developed, but have the potential to deliver fast, inexpensive and simple full blood cell analysis systems that could be used routinely in clinical practice. We demonstrate a microfluidic single cell impedance cytometer that performs a white blood cell differential count. The device consists of a microfluidic chip with micro-electrodes that measure the impedance of single cells at two frequencies. Human blood, treated with saponin/formic acid to lyse erythrocytes, flows through the device and a complete blood count is performed in a few minutes. Verification of cell dielectric parameters was performed by simultaneously measuring fluorescence from CD antibody-conjugated cells. This enabled direct correlation of impedance signals from individual cells with phenotype. Tests with patient samples showed 95\% correlation against commercial (optical/Coulter) blood analysis equipment, demonstrating the potential clinical utility of the impedance microcytometer for a point-of-care blood analysis system.}, author = {Holmes, David and Pettigrew, David and Reccius, Christian H. and Gwyer, James D. and van Berkel, Cees and Holloway, Judith and Davies, Donna E. and Morgan, Hywel}, citeulike-article-id = {5730503}, citeulike-linkout-0 = {http://dx.doi.org/10.1039/b910053a}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19789739}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19789739}, citeulike-linkout-3 = {http://www.rsc.org/Publishing/Journals/article.asp?doi=b910053a}, day = {21}, doi = {10.1039/b910053a}, issn = {1473-0197}, journal = {Lab on a chip}, keywords = {cytometry, microfluidics}, month = {October}, number = {20}, pages = {2881--2889}, posted-at = {2009-09-06 23:33:29}, priority = {2}, publisher = {The Royal Society of Chemistry}, title = {Leukocyte analysis and differentiation using high speed microfluidic single cell impedance cytometry.}, url = {http://dx.doi.org/10.1039/b910053a}, volume = {9}, year = {2009} }

TY - JOUR ID - citeulike:5730503 L3 - citeulike-article-id:5730503 N2 - Miniature high speed label-free cell analysis systems have yet to be developed, but have the potential to deliver fast, inexpensive and simple full blood cell analysis systems that could be used routinely in clinical practice. We demonstrate a microfluidic single cell impedance cytometer that performs a white blood cell differential count. The device consists of a microfluidic chip with micro-electrodes that measure the impedance of single cells at two frequencies. Human blood, treated with saponin/formic acid to lyse erythrocytes, flows through the device and a complete blood count is performed in a few minutes. Verification of cell dielectric parameters was performed by simultaneously measuring fluorescence from CD antibody-conjugated cells. This enabled direct correlation of impedance signals from individual cells with phenotype. Tests with patient samples showed 95% correlation against commercial (optical/Coulter) blood analysis equipment, demonstrating the potential clinical utility of the impedance microcytometer for a point-of-care blood analysis system. IS - 20 JF - Lab on a chip SN - 1473-0197 EP - 2889 TI - Leukocyte analysis and differentiation using high speed microfluidic single cell impedance cytometry. PB - The Royal Society of Chemistry VL - 9 SP - 2881 KW - cytometry KW - microfluidics AU - Holmes, David AU - Pettigrew, David AU - Reccius, Christian AU - Gwyer, James AU - van Berkel, Cees AU - Holloway, Judith AU - Davies, Donna AU - Morgan, Hywel PY - 2009/10/21/ UR - http://dx.doi.org/10.1039/b910053a ER -