Chemomechanical Coupling without ATP: The Source of Energy for Motility and Chemotaxis in Bacteria

@article{Larsen1974, abstract = {The source of energy for bacterial motility is the intermediate in oxidative phosphorylation, not ATP directly. For chemotaxis, however, there is an additional requirement, presumably ATP. These conclusions are based on the following findings. (i) Unlike their parents, mutants of Escherichia coli and Salmonella typhimurium that are blocked in the conversion of ATP to the intermediate of oxidative phosphorylation failed to swim anaerobically, even when they produced ATP. When respiration was restored to the mutants, motility was simultaneously restored. (ii) Carbonylcyanide m-chlorophenyl-hydrazone, which uncouples oxidative phosphorylation, completely inhibited motility even though ATP remained present. (iii) Arsenate did not inhibit motility in the presence of an oxidizable substrate, though it did reduce ATP levels to less than 0.3\%. (iv) Arsenate completely inhibited chemotaxis under conditions where motility was normal. 10.1073/pnas.71.4.1239}, author = {Larsen, Steven H. and Adler, Julius and Gargus, Jay J. and Hogg, Robert W. }, citeulike-article-id = {2746750}, doi = {10.1073/pnas.71.4.1239}, journal = {Proceedings of the National Academy of Sciences}, keywords = {bacteria\_flagella\_project, biophysics}, month = {April}, number = {4}, pages = {1239--1243}, posted-at = {2008-05-02 19:19:00}, priority = {2}, title = {Chemomechanical Coupling without ATP: The Source of Energy for Motility and Chemotaxis in Bacteria}, url = {http://dx.doi.org/10.1073/pnas.71.4.1239}, volume = {71}, year = {1974} }

TY - JOUR ID - Larsen1974 L3 - citeulike-article-id:2746750 TI - Chemomechanical Coupling without ATP: The Source of Energy for Motility and Chemotaxis in Bacteria JF - Proceedings of the National Academy of Sciences VL - 71 IS - 4 SP - 1239 EP - 1243 N2 - The source of energy for bacterial motility is the intermediate in oxidative phosphorylation, not ATP directly. For chemotaxis, however, there is an additional requirement, presumably ATP. These conclusions are based on the following findings. (i) Unlike their parents, mutants of Escherichia coli and Salmonella typhimurium that are blocked in the conversion of ATP to the intermediate of oxidative phosphorylation failed to swim anaerobically, even when they produced ATP. When respiration was restored to the mutants, motility was simultaneously restored. (ii) Carbonylcyanide m-chlorophenyl-hydrazone, which uncouples oxidative phosphorylation, completely inhibited motility even though ATP remained present. (iii) Arsenate did not inhibit motility in the presence of an oxidizable substrate, though it did reduce ATP levels to less than 0.3%. (iv) Arsenate completely inhibited chemotaxis under conditions where motility was normal. 10.1073/pnas.71.4.1239 KW - bacteria_flagella_project KW - biophysics AU - Larsen, Steven AU - Adler, Julius AU - Gargus, Jay AU - Hogg, Robert PY - 1974/04/15/ UR - http://dx.doi.org/10.1073/pnas.71.4.1239 ER -