Functional identification of tumor-suppressor genes through an in vivo RNA interference screen in a mouse lymphoma model. Export

@article{citeulike:5917513, abstract = {Short hairpin RNAs (shRNAs) capable of stably suppressing gene function by RNA interference (RNAi) can mimic tumor-suppressor-gene loss in mice. By selecting for shRNAs capable of accelerating lymphomagenesis in a well-characterized mouse lymphoma model, we identified over ten candidate tumor suppressors, including Sfrp1, Numb, Mek1, and Angiopoietin 2. Several components of the DNA damage response machinery were also identified, including Rad17, which acts as a haploinsufficient tumor suppressor that responds to oncogenic stress and whose loss is associated with poor prognosis in human patients. Our results emphasize the utility of in vivo RNAi screens, identify and validate a diverse set of tumor suppressors, and have therapeutic implications.}, author = {Bric, Anka and Miething, Cornelius and Bialucha, Carl Uli U. and Scuoppo, Claudio and Zender, Lars and Krasnitz, Alexander and Xuan, Zhenyu and Zuber, Johannes and Wigler, Michael and Hicks, James and McCombie, Richard W. and Hemann, Michael T. and Hannon, Gregory J. and Powers, Scott and Lowe, Scott W.}, citeulike-article-id = {5917513}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.ccr.2009.08.015}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S1535610809002621}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19800577}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19800577}, day = {6}, doi = {10.1016/j.ccr.2009.08.015}, issn = {1878-3686}, journal = {Cancer cell}, keywords = {genetics, medicine, mesophysics}, month = {October}, number = {4}, pages = {324--335}, posted-at = {2009-10-11 14:50:22}, priority = {2}, title = {Functional identification of tumor-suppressor genes through an in vivo RNA interference screen in a mouse lymphoma model.}, url = {http://dx.doi.org/10.1016/j.ccr.2009.08.015}, volume = {16}, year = {2009} }

TY - JOUR ID - citeulike:5917513 L3 - citeulike-article-id:5917513 N2 - Short hairpin RNAs (shRNAs) capable of stably suppressing gene function by RNA interference (RNAi) can mimic tumor-suppressor-gene loss in mice. By selecting for shRNAs capable of accelerating lymphomagenesis in a well-characterized mouse lymphoma model, we identified over ten candidate tumor suppressors, including Sfrp1, Numb, Mek1, and Angiopoietin 2. Several components of the DNA damage response machinery were also identified, including Rad17, which acts as a haploinsufficient tumor suppressor that responds to oncogenic stress and whose loss is associated with poor prognosis in human patients. Our results emphasize the utility of in vivo RNAi screens, identify and validate a diverse set of tumor suppressors, and have therapeutic implications. IS - 4 JF - Cancer cell SN - 1878-3686 EP - 335 TI - Functional identification of tumor-suppressor genes through an in vivo RNA interference screen in a mouse lymphoma model. VL - 16 SP - 324 KW - genetics KW - medicine KW - mesophysics AU - Bric, Anka AU - Miething, Cornelius AU - Bialucha, Carl Uli AU - Scuoppo, Claudio AU - Zender, Lars AU - Krasnitz, Alexander AU - Xuan, Zhenyu AU - Zuber, Johannes AU - Wigler, Michael AU - Hicks, James AU - McCombie, Richard AU - Hemann, Michael AU - Hannon, Gregory AU - Powers, Scott AU - Lowe, Scott PY - 2009/10/06/ UR - http://dx.doi.org/10.1016/j.ccr.2009.08.015 ER -