Designed α-Helical Tectons for Constructing Multicomponent Synthetic Biological Systems Export

@article{Bromley2009, abstract = {One possible route to develop new synthetic-biological systems is to assemble discrete nanoscale objects from programmed peptide-based building blocks. We describe an algorithm to design such blocks based on the coiled-coil protein-folding motif. The success of the algorithm is demonstrated by the production of six peptides that form three target parallel, blunted-ended heterodimers in preference to any of the other promiscuous pairings and alternate configurations, for example, homodimers, sticky-ended assemblies, and antiparallel arrangements. The peptides were linked to promote the assembly of larger, defined nanoscale rods, thus demonstrating that targeted peptide−peptide interactions can be specified in complex mixtures.}, author = {Bromley, Elizabeth H. C. and Sessions, Richard B. and Thomson, Andrew R. and Woolfson, Derek N.}, citeulike-article-id = {4142845}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/ja804231a}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/ja804231a}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19115943}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19115943}, day = {28}, doi = {10.1021/ja804231a}, issn = {0002-7863}, journal = {Journal of the American Chemical Society}, keywords = {circular-dichroism, coiled-coil, peptide-design, synthetic-biology}, month = {January}, number = {3}, pages = {928--930}, posted-at = {2009-09-23 14:07:20}, priority = {0}, title = {Designed α-Helical Tectons for Constructing Multicomponent Synthetic Biological Systems}, url = {http://dx.doi.org/10.1021/ja804231a}, volume = {131}, year = {2009} }

TY - JOUR ID - Bromley2009 L3 - citeulike-article-id:4142845 N2 - One possible route to develop new synthetic-biological systems is to assemble discrete nanoscale objects from programmed peptide-based building blocks. We describe an algorithm to design such blocks based on the coiled-coil protein-folding motif. The success of the algorithm is demonstrated by the production of six peptides that form three target parallel, blunted-ended heterodimers in preference to any of the other promiscuous pairings and alternate configurations, for example, homodimers, sticky-ended assemblies, and antiparallel arrangements. The peptides were linked to promote the assembly of larger, defined nanoscale rods, thus demonstrating that targeted peptide−peptide interactions can be specified in complex mixtures. IS - 3 JF - Journal of the American Chemical Society SN - 0002-7863 EP - 930 TI - Designed α-Helical Tectons for Constructing Multicomponent Synthetic Biological Systems VL - 131 SP - 928 KW - circular-dichroism KW - coiled-coil KW - peptide-design KW - synthetic-biology AU - Bromley, Elizabeth AU - Sessions, Richard AU - Thomson, Andrew AU - Woolfson, Derek PY - 2009/01/28/ UR - http://dx.doi.org/10.1021/ja804231a ER -