A vesicle bioreactor as a step toward an artificial cell assembly. Export

@article{noireaux2004, abstract = {An Escherichia coli cell-free expression system is encapsulated in a phospholipid vesicle to build a cell-like bioreactor. Large unilamellar vesicles containing extracts are produced in an oil-extract emulsion. To form a bilayer the vesicles are transferred into a feeding solution that contains ribonucleotides and amino acids. Transcription-translation of plasmid genes is isolated in the vesicles. Whereas in bulk solution expression of enhanced GFP stops after 2 h, inside the vesicle permeability of the membrane to the feeding solution prolongs the expression for up to 5 h. To solve the energy and material limitations and increase the capacity of the reactor, the alpha-hemolysin pore protein from Staphylococcus aureus is expressed inside the vesicle to create a selective permeability for nutrients. The reactor can then sustain expression for up to 4 days with a protein production of 30 microM after 4 days. Oxygen diffusion and osmotic pressure are critical parameters to maintain expression and avoid vesicle burst.}, address = {Center for Studies in Physics and Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021.}, author = {Noireaux, Vincent and Libchaber, Albert}, citeulike-article-id = {4262}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0408236101}, citeulike-linkout-1 = {http://www.pnas.org/cgi/content/abstract/101/51/17669}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/15591347}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=15591347}, day = {10}, doi = {10.1073/pnas.0408236101}, issn = {0027-8424}, journal = {Proc Natl Acad Sci U S A}, keywords = {amphiphiles, encapsulation, genetic-engineering, lipid, molecular-biology, proteins, self-assembly, synthetic-biology, vesicle}, month = {December}, posted-at = {2008-07-16 15:38:49}, priority = {2}, title = {A vesicle bioreactor as a step toward an artificial cell assembly.}, url = {http://dx.doi.org/10.1073/pnas.0408236101}, year = {2004} }

TY - JOUR ID - noireaux2004 L3 - citeulike-article-id:4262 N2 - An Escherichia coli cell-free expression system is encapsulated in a phospholipid vesicle to build a cell-like bioreactor. Large unilamellar vesicles containing extracts are produced in an oil-extract emulsion. To form a bilayer the vesicles are transferred into a feeding solution that contains ribonucleotides and amino acids. Transcription-translation of plasmid genes is isolated in the vesicles. Whereas in bulk solution expression of enhanced GFP stops after 2 h, inside the vesicle permeability of the membrane to the feeding solution prolongs the expression for up to 5 h. To solve the energy and material limitations and increase the capacity of the reactor, the alpha-hemolysin pore protein from Staphylococcus aureus is expressed inside the vesicle to create a selective permeability for nutrients. The reactor can then sustain expression for up to 4 days with a protein production of 30 microM after 4 days. Oxygen diffusion and osmotic pressure are critical parameters to maintain expression and avoid vesicle burst. JF - Proc Natl Acad Sci U S A SN - 0027-8424 AD - Center for Studies in Physics and Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021. TI - A vesicle bioreactor as a step toward an artificial cell assembly. KW - amphiphiles KW - encapsulation KW - genetic-engineering KW - lipid KW - molecular-biology KW - proteins KW - self-assembly KW - synthetic-biology KW - vesicle AU - Noireaux, Vincent AU - Libchaber, Albert PY - 2004/12/10/ UR - http://dx.doi.org/10.1073/pnas.0408236101 ER -