Membrane Fusion of Giant Unilamellar Vesicles of Neutral Phospholipid Membranes Induced by La3+ Export

@article{Tanaka2004, abstract = {Membrane fusions of vesicles of biomembranes play various important roles in cells, but their mechanisms are unclear and controversial. In the present study, we found that 30 muM to 1 mM La3+ induced membrane fusion of two giant unilamellar vesicles (GUVs) composed of a mixture of dioleoylphosphatidylcholine (DOPC) and dipalmitoleoylphosphatidylethanolamine (DPOPE). We succeeded in observing a process of this membrane fusion in detail. First, two GUVs became strongly associated, with a partition membrane between them composed of two bilayers, one from each GUV. Then, the partition membrane was suddenly broken at one site on its edge. The area of this breakage site gradually spread, until it was completely separated from the GUV to complete the membrane fusion. Here, we propose a new model (i.e., the partition breakage model) for the mechanism of La3+-induced membrane fusion of GUVs.}, author = {Tanaka, Tomoki and Yamazaki, Masahito}, citeulike-article-id = {4785817}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/la049681s}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/la049681s}, day = {1}, doi = {10.1021/la049681s}, journal = {Langmuir}, keywords = {amphiphiles, membrane-fusion, membranes, vesicle}, month = {June}, number = {13}, pages = {5160--5164}, posted-at = {2009-06-09 12:24:28}, priority = {2}, title = {Membrane Fusion of Giant Unilamellar Vesicles of Neutral Phospholipid Membranes Induced by La3+}, url = {http://dx.doi.org/10.1021/la049681s}, volume = {20}, year = {2004} }

TY - JOUR ID - Tanaka2004 L3 - citeulike-article-id:4785817 N2 - Membrane fusions of vesicles of biomembranes play various important roles in cells, but their mechanisms are unclear and controversial. In the present study, we found that 30 muM to 1 mM La3+ induced membrane fusion of two giant unilamellar vesicles (GUVs) composed of a mixture of dioleoylphosphatidylcholine (DOPC) and dipalmitoleoylphosphatidylethanolamine (DPOPE). We succeeded in observing a process of this membrane fusion in detail. First, two GUVs became strongly associated, with a partition membrane between them composed of two bilayers, one from each GUV. Then, the partition membrane was suddenly broken at one site on its edge. The area of this breakage site gradually spread, until it was completely separated from the GUV to complete the membrane fusion. Here, we propose a new model (i.e., the partition breakage model) for the mechanism of La3+-induced membrane fusion of GUVs. IS - 13 JF - Langmuir EP - 5164 TI - Membrane Fusion of Giant Unilamellar Vesicles of Neutral Phospholipid Membranes Induced by La3+ VL - 20 SP - 5160 KW - amphiphiles KW - membrane-fusion KW - membranes KW - vesicle AU - Tanaka, Tomoki AU - Yamazaki, Masahito PY - 2004/06/01/ UR - http://dx.doi.org/10.1021/la049681s ER -