Polyhydroxyalkanoate Film Formation and Synthase Activity During In Vitro and In Situ Polymerization on Hydrophobic Surfaces Export

@article{Sato2008, abstract = {PMID: 18771315 In vitro and in situ enzymatic polymerization of polyhydroxyalkanoate (PHA) on two hydrophobic surfaces, a highly oriented pyrolytic graphite (HOPG) and an alkanethiol self-assembled monolayer (SAM), was studied by atomic force microscopy (AFM) and quartz crystal microbalance (QCM), using purified Ralstonia eutropha PHA synthase (PhaCRe) as a biocatalyst. (R)-Specific enoyl-CoA hydratase was used to prepare R-enantiomer monomers [(R)-3-hydroxyacyl-CoA] with an acyl chain length of 4−6 carbon atoms. PHA homopolymers with different side-chain lengths, poly[(R)-3-hydroxybutyrate] [P(3HB)] and poly[(R)-3-hydroxyvalerate] [P(3HV)] were successfully synthesized from such R-enantiomer monomers on HOPG substrates. After the reaction, the surface morphologies were analyzed by AFM, revealing a nanometer thick PHA film. The same biochemical polymerization process was observed on an alkanethiol (C18) SAM surface fabricated on a gold electrode using QCM. This analysis showed that a complex sequence of PhaCRe adsorption and PHA polymerization has occurred on the hydrophobic surface. On the basis of these observations, the possible mechanisms of the PhaCRe-catalyzed polymerization reaction on the surface of hydrophobic substrates are proposed.}, author = {Sato, Shun and Ono, Yusuke and Mochiyama, Yukiko and Sivaniah, Easan and Kikkawa, Yoshihiro and Sudesh, Kumar and Hiraishi, Tomohiro and Doi, Yoshiharu and Abe, Hideki and Tsuge, Takeharu}, citeulike-article-id = {5864991}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/bm800566s}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/bm800566s}, day = {13}, doi = {10.1021/bm800566s}, journal = {Biomacromolecules}, keywords = {bioplastics, enzymes, pha, polymers}, month = {October}, number = {10}, pages = {2811--2818}, posted-at = {2009-10-01 01:32:17}, priority = {2}, title = {Polyhydroxyalkanoate Film Formation and Synthase Activity During In Vitro and In Situ Polymerization on Hydrophobic Surfaces}, url = {http://dx.doi.org/10.1021/bm800566s}, volume = {9}, year = {2008} }

TY - JOUR ID - Sato2008 L3 - citeulike-article-id:5864991 N2 - PMID: 18771315 In vitro and in situ enzymatic polymerization of polyhydroxyalkanoate (PHA) on two hydrophobic surfaces, a highly oriented pyrolytic graphite (HOPG) and an alkanethiol self-assembled monolayer (SAM), was studied by atomic force microscopy (AFM) and quartz crystal microbalance (QCM), using purified Ralstonia eutropha PHA synthase (PhaCRe) as a biocatalyst. (R)-Specific enoyl-CoA hydratase was used to prepare R-enantiomer monomers [(R)-3-hydroxyacyl-CoA] with an acyl chain length of 4−6 carbon atoms. PHA homopolymers with different side-chain lengths, poly[(R)-3-hydroxybutyrate] [P(3HB)] and poly[(R)-3-hydroxyvalerate] [P(3HV)] were successfully synthesized from such R-enantiomer monomers on HOPG substrates. After the reaction, the surface morphologies were analyzed by AFM, revealing a nanometer thick PHA film. The same biochemical polymerization process was observed on an alkanethiol (C18) SAM surface fabricated on a gold electrode using QCM. This analysis showed that a complex sequence of PhaCRe adsorption and PHA polymerization has occurred on the hydrophobic surface. On the basis of these observations, the possible mechanisms of the PhaCRe-catalyzed polymerization reaction on the surface of hydrophobic substrates are proposed. IS - 10 JF - Biomacromolecules EP - 2818 TI - Polyhydroxyalkanoate Film Formation and Synthase Activity During In Vitro and In Situ Polymerization on Hydrophobic Surfaces VL - 9 SP - 2811 KW - bioplastics KW - enzymes KW - pha KW - polymers AU - Sato, Shun AU - Ono, Yusuke AU - Mochiyama, Yukiko AU - Sivaniah, Easan AU - Kikkawa, Yoshihiro AU - Sudesh, Kumar AU - Hiraishi, Tomohiro AU - Doi, Yoshiharu AU - Abe, Hideki AU - Tsuge, Takeharu PY - 2008/10/13/ UR - http://dx.doi.org/10.1021/bm800566s ER -