Photocurrents Generated by Bacteriorhodopsin Adsorbed on Nano-Black Lipid Membranes Export

@article{Horn2005, abstract = {Purple membranes were adsorbed on freestanding lipid bilayers, termed nano-black lipid membranes (nano-BLMs), suspending the pores of porous alumina substrates with average pore diameters of 280nm. Nano-BLMs were obtained by first coating the upper surface of the highly ordered porous alumina substrates with a thin gold layer followed by chemisorption of 1,2-dipalmitoyl-sn-glycero-3-phosphothioethanol and subsequent addition of a droplet of 1,2-diphytanoyl-sn-glycero-3-phosphocholine and octadecylamine dissolved in n-decane onto the hydrophobic submonolayer. By means of impedance spectroscopy, the quality of the nano-BLMs was verified. The electrical parameters confirm the formation of single lipid bilayers with high membrane resistances covering the porous matrix. Adsorption of purple membranes on the nano-BLMs was followed by recording the photocurrents generated by bacteriorhodopsin upon continuous light illumination. The membrane system exhibits a very high long-term stability with the advantage that not only transient but also stationary currents are recordable. By adding the proton ionophore carbonyl cyanide-m-chlorophenylhydrazone the conductivity of the nano-BLMs increases, resulting in a higher stationary current, which proves that proton conductance occurs across the nano-BLMs.}, author = {Horn, Christian and Steinem, Claudia}, citeulike-article-id = {6057026}, citeulike-linkout-0 = {http://www.cell.com/biophysj/abstract/S0006-3495(05)72753-3}, day = {1}, keywords = {bacteriorhodopsin, biomolecular-electronics, biophysics, membranes, photophysics, photovoltaics}, month = {August}, number = {2}, pages = {1046--1054}, posted-at = {2009-11-02 17:49:46}, priority = {2}, title = {Photocurrents Generated by Bacteriorhodopsin Adsorbed on Nano-Black Lipid Membranes}, url = {http://www.cell.com/biophysj/abstract/S0006-3495(05)72753-3}, volume = {89}, year = {2005} }

TY - JOUR ID - Horn2005 L3 - citeulike-article-id:6057026 N2 - Purple membranes were adsorbed on freestanding lipid bilayers, termed nano-black lipid membranes (nano-BLMs), suspending the pores of porous alumina substrates with average pore diameters of 280nm. Nano-BLMs were obtained by first coating the upper surface of the highly ordered porous alumina substrates with a thin gold layer followed by chemisorption of 1,2-dipalmitoyl-sn-glycero-3-phosphothioethanol and subsequent addition of a droplet of 1,2-diphytanoyl-sn-glycero-3-phosphocholine and octadecylamine dissolved in n-decane onto the hydrophobic submonolayer. By means of impedance spectroscopy, the quality of the nano-BLMs was verified. The electrical parameters confirm the formation of single lipid bilayers with high membrane resistances covering the porous matrix. Adsorption of purple membranes on the nano-BLMs was followed by recording the photocurrents generated by bacteriorhodopsin upon continuous light illumination. The membrane system exhibits a very high long-term stability with the advantage that not only transient but also stationary currents are recordable. By adding the proton ionophore carbonyl cyanide-m-chlorophenylhydrazone the conductivity of the nano-BLMs increases, resulting in a higher stationary current, which proves that proton conductance occurs across the nano-BLMs. IS - 2 EP - 1054 TI - Photocurrents Generated by Bacteriorhodopsin Adsorbed on Nano-Black Lipid Membranes VL - 89 SP - 1046 KW - bacteriorhodopsin KW - biomolecular-electronics KW - biophysics KW - membranes KW - photophysics KW - photovoltaics AU - Horn, Christian AU - Steinem, Claudia PY - 2005/08/01/ UR - http://www.cell.com/biophysj/abstract/S0006-3495(05)72753-3 ER -