Oriented purple-membrane films as a probe for studies of the mechanism of bacteriorhodopsin functioning. II. Photoelectric processes Export

@article{Kononenko1987, abstract = {Photoelectric processes have been investigated in dry orderly oriented preparations of purple membranes from Halobacterium halobium under both continuous light and flash excitation. An electrophoretic sedimentation on Al, Cu, Fe, Ni, Pt and SnO 2 substrates was used to obtain orderly oriented purple-membrane films. The photoelectric response of the purple-membrane film is the sum of a light-induced 'displacement' current and a constant steady-state current, the proportion between the two depending upon the chemical nature of the electrodes and humidity of the film. With a high humidity, the steady-state photocurrent is correlated with the reactivity of the cathode metal (toward H + reduction reaction. A correlation is found to exist between the kinetics of photopotential rise and decay and formation and decay of the K 630 and M 412 intermediates of the bacteriorhodopsin photocycle at temperatures ranging from 293 to 83 K, indicating the electrogenic nature of these intermediates. In purple-membrane films deposited on an SnO 2 substrate, a correlation exists between the 'dark' potential and electron work function of the second electrode.}, author = {Kononenko, A. and Lukashev, E. and Chamorovsky, S. and Maximychev, A. and Timashev, S. and Chekulaeva, L. and Rubin, A. and Paschenko, V.}, citeulike-article-id = {6059925}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/0005-2728(87)90247-7}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/0005272887902477}, day = {09}, doi = {10.1016/0005-2728(87)90247-7}, issn = {00052728}, journal = {Biochimica et Biophysica Acta (BBA) - Bioenergetics}, keywords = {bacteriorhodopsin, biophysics, photophysics}, month = {June}, number = {1}, pages = {56--67}, posted-at = {2009-11-03 11:29:41}, priority = {2}, title = {Oriented purple-membrane films as a probe for studies of the mechanism of bacteriorhodopsin functioning. II. Photoelectric processes}, url = {http://dx.doi.org/10.1016/0005-2728(87)90247-7}, volume = {892}, year = {1987} }

TY - JOUR ID - Kononenko1987 L3 - citeulike-article-id:6059925 N2 - Photoelectric processes have been investigated in dry orderly oriented preparations of purple membranes from Halobacterium halobium under both continuous light and flash excitation. An electrophoretic sedimentation on Al, Cu, Fe, Ni, Pt and SnO 2 substrates was used to obtain orderly oriented purple-membrane films. The photoelectric response of the purple-membrane film is the sum of a light-induced ‘displacement’ current and a constant steady-state current, the proportion between the two depending upon the chemical nature of the electrodes and humidity of the film. With a high humidity, the steady-state photocurrent is correlated with the reactivity of the cathode metal (toward H + reduction reaction. A correlation is found to exist between the kinetics of photopotential rise and decay and formation and decay of the K 630 and M 412 intermediates of the bacteriorhodopsin photocycle at temperatures ranging from 293 to 83 K, indicating the electrogenic nature of these intermediates. In purple-membrane films deposited on an SnO 2 substrate, a correlation exists between the ‘dark’ potential and electron work function of the second electrode. IS - 1 JF - Biochimica et Biophysica Acta (BBA) - Bioenergetics SN - 00052728 EP - 67 TI - Oriented purple-membrane films as a probe for studies of the mechanism of bacteriorhodopsin functioning. II. Photoelectric processes VL - 892 SP - 56 KW - bacteriorhodopsin KW - biophysics KW - photophysics AU - Kononenko, A AU - Lukashev, E AU - Chamorovsky, S AU - Maximychev, A AU - Timashev, S AU - Chekulaeva, L AU - Rubin, A AU - Paschenko, V PY - 1987/06/09/ UR - http://dx.doi.org/10.1016/0005-2728(87)90247-7 ER -