The effect of charged lipids on bacteriorhodopsin membrane reconstitution and its photochemical activities Export

@article{Wang2008, abstract = {Bacteriorhodopsin (BR) was reconstituted into artificial lipid membrane containing various charged lipid compositions. The proton pumping activity of BR under flash and continuous illumination, proton permeability across membrane, as well as the decay kinetics of the photocycle intermediate M 412 were studied. The results showed that lipid charges would significantly affect the orientation of BR inserted into lipid membranes. In liposomes containing anionic lipids, BRs were more likely to take natural orientation as in living cells. In neutral or positively charged liposomes, most BRs were reversely assembled, assuming an inside out orientation. Moreover, the lipids charges also affect BR's M intermediate kinetics, especially the slow component in M intermediate decay. The half-life M 412s increased significantly in BRs in liposomes containing cationic lipids, while decreased in those in anionic liposomes.}, author = {Wang, Z. and Bai, J. and Xu, Y.}, citeulike-article-id = {6075578}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.bbrc.2008.04.141}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0006291X08008553}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18460340}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18460340}, day = {11}, doi = {10.1016/j.bbrc.2008.04.141}, issn = {0006291X}, journal = {Biochemical and Biophysical Research Communications}, keywords = {bacteriorhodopsin, vesicle}, month = {July}, number = {4}, pages = {814--817}, posted-at = {2009-11-05 17:20:16}, priority = {0}, title = {The effect of charged lipids on bacteriorhodopsin membrane reconstitution and its photochemical activities}, url = {http://dx.doi.org/10.1016/j.bbrc.2008.04.141}, volume = {371}, year = {2008} }

TY - JOUR ID - Wang2008 L3 - citeulike-article-id:6075578 N2 - Bacteriorhodopsin (BR) was reconstituted into artificial lipid membrane containing various charged lipid compositions. The proton pumping activity of BR under flash and continuous illumination, proton permeability across membrane, as well as the decay kinetics of the photocycle intermediate M 412 were studied. The results showed that lipid charges would significantly affect the orientation of BR inserted into lipid membranes. In liposomes containing anionic lipids, BRs were more likely to take natural orientation as in living cells. In neutral or positively charged liposomes, most BRs were reversely assembled, assuming an inside out orientation. Moreover, the lipids charges also affect BR’s M intermediate kinetics, especially the slow component in M intermediate decay. The half-life M 412s increased significantly in BRs in liposomes containing cationic lipids, while decreased in those in anionic liposomes. IS - 4 JF - Biochemical and Biophysical Research Communications SN - 0006291X EP - 817 TI - The effect of charged lipids on bacteriorhodopsin membrane reconstitution and its photochemical activities VL - 371 SP - 814 KW - bacteriorhodopsin KW - vesicle AU - Wang, Z AU - Bai, J AU - Xu, Y PY - 2008/07/11/ UR - http://dx.doi.org/10.1016/j.bbrc.2008.04.141 ER -