Oriented Purple Membrane Monolayers Covalently Attached to Gold by Multiple Thiole Linkages Analyzed by Single Molecule Force Spectroscopy Export

@article{Schranz2007, abstract = {PMID: 17887782 Highly oriented monolayers of bacteriorhodopsin (BR) in purple membrane (PM) form are obtained by the reaction of BR-Q3C, where a cysteine was introduced into the N-terminal region, with a gold surface. Single molecule force spectroscopy was used to show that about 50\% of the BRs are covalently bound to the surface. The linkage between the cysteine and the gold causes an additional characteristic peak in the forcedistance curves to appear. Because several thousand cysteinegold bonds exist between each PM patch and the surface, the PM is irreversibly bound. Such oriented PM monolayers may serve as an interface between metal surfaces and biomaterials, which may be linked to the PM surface chemically. Photoelectric applications of BR will benefit from the high degree of orientation obtained by this method.}, author = {Schranz, Michael and Noll, Frank and Hampp, Norbert}, citeulike-article-id = {6075788}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/la7019928}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/la7019928}, day = {1}, doi = {10.1021/la7019928}, journal = {Langmuir}, keywords = {bacteriorhodopsin, force-spectroscopy, membranes}, month = {October}, number = {22}, pages = {11134--11138}, posted-at = {2009-11-05 17:51:43}, priority = {2}, title = {Oriented Purple Membrane Monolayers Covalently Attached to Gold by Multiple Thiole Linkages Analyzed by Single Molecule Force Spectroscopy}, url = {http://dx.doi.org/10.1021/la7019928}, volume = {23}, year = {2007} }

TY - JOUR ID - Schranz2007 L3 - citeulike-article-id:6075788 N2 - PMID: 17887782 Highly oriented monolayers of bacteriorhodopsin (BR) in purple membrane (PM) form are obtained by the reaction of BR-Q3C, where a cysteine was introduced into the N-terminal region, with a gold surface. Single molecule force spectroscopy was used to show that about 50% of the BRs are covalently bound to the surface. The linkage between the cysteine and the gold causes an additional characteristic peak in the forcedistance curves to appear. Because several thousand cysteinegold bonds exist between each PM patch and the surface, the PM is irreversibly bound. Such oriented PM monolayers may serve as an interface between metal surfaces and biomaterials, which may be linked to the PM surface chemically. Photoelectric applications of BR will benefit from the high degree of orientation obtained by this method. IS - 22 JF - Langmuir EP - 11138 TI - Oriented Purple Membrane Monolayers Covalently Attached to Gold by Multiple Thiole Linkages Analyzed by Single Molecule Force Spectroscopy VL - 23 SP - 11134 KW - bacteriorhodopsin KW - force-spectroscopy KW - membranes AU - Schranz, Michael AU - Noll, Frank AU - Hampp, Norbert PY - 2007/10/01/ UR - http://dx.doi.org/10.1021/la7019928 ER -