The directed cooperative assembly of proteorhodopsin into 2D and 3D polarized arrays Export

@article{Liang2007, abstract = {10.1073/pnas.0702336104 Proteorhodopsin is the membrane protein used by marine bacterioplankton as a light-driven proton pump. Here, we describe a rapid cooperative assembly process directed by universal electrostatic interactions that spontaneously organizes proteorhodopsin molecules into ordered arrays with well defined orientation and packing density. We demonstrate the charge density-matching mechanism that selectively controls the assembly process. The interactions among different components in the system are tuned by varying their charge densities to yield different organized transmembrane protein arrays: () a bacteriorhodopsin purple membrane-like structure where proteorhodopsin molecules are cooperatively arranged with charged lipids into a 2D hexagonal lattice; () selected liquid-crystalline states in which crystalline lamellae made up of the coassembled proteorhodopsin and charged lipid molecules are coupled three-dimensionally with polarized proteorhodopsin orientation persisting through the macroscopic scale. Understanding this rapid electrostatically driven assembly process sheds light on organizing membrane proteins in general, which is a prerequisite for membrane protein structural and mechanistic studies as well as applications.}, author = {Liang, Hongjun and Whited, Gregg and Nguyen, Chi and Stucky, Galen D.}, citeulike-article-id = {6075811}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0702336104}, citeulike-linkout-1 = {http://www.pnas.org/content/104/20/8212.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/104/20/8212.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/17488827}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=17488827}, day = {15}, doi = {10.1073/pnas.0702336104}, journal = {Proceedings of the National Academy of Sciences}, keywords = {bacteriorhodopsin, biophysics, membranes, self-assembly}, month = {May}, number = {20}, pages = {8212--8217}, posted-at = {2009-11-05 17:55:19}, priority = {2}, title = {The directed cooperative assembly of proteorhodopsin into 2D and 3D polarized arrays}, url = {http://dx.doi.org/10.1073/pnas.0702336104}, volume = {104}, year = {2007} }

TY - JOUR ID - Liang2007 L3 - citeulike-article-id:6075811 N2 - 10.1073/pnas.0702336104 Proteorhodopsin is the membrane protein used by marine bacterioplankton as a light-driven proton pump. Here, we describe a rapid cooperative assembly process directed by universal electrostatic interactions that spontaneously organizes proteorhodopsin molecules into ordered arrays with well defined orientation and packing density. We demonstrate the charge density-matching mechanism that selectively controls the assembly process. The interactions among different components in the system are tuned by varying their charge densities to yield different organized transmembrane protein arrays: () a bacteriorhodopsin purple membrane-like structure where proteorhodopsin molecules are cooperatively arranged with charged lipids into a 2D hexagonal lattice; () selected liquid-crystalline states in which crystalline lamellae made up of the coassembled proteorhodopsin and charged lipid molecules are coupled three-dimensionally with polarized proteorhodopsin orientation persisting through the macroscopic scale. Understanding this rapid electrostatically driven assembly process sheds light on organizing membrane proteins in general, which is a prerequisite for membrane protein structural and mechanistic studies as well as applications. IS - 20 JF - Proceedings of the National Academy of Sciences EP - 8217 TI - The directed cooperative assembly of proteorhodopsin into 2D and 3D polarized arrays VL - 104 SP - 8212 KW - bacteriorhodopsin KW - biophysics KW - membranes KW - self-assembly AU - Liang, Hongjun AU - Whited, Gregg AU - Nguyen, Chi AU - Stucky, Galen PY - 2007/05/15/ UR - http://dx.doi.org/10.1073/pnas.0702336104 ER -