Investigation of the orientation of purple membrane sheets in Langmuir-Blodgett films by a quartz crystal microbalance. Export

@article{Lu1999, abstract = {By means of the quartz crystal microbalance (QCM), a convenient method was developed to determine the degree of orientation of purple membrane (PM) sheets on the air/water interface. Langmuir-Blodgett films from both wild-type and SH-mutant PM (bR D36C) were vertically deposited on the surface of gold-sputtered AT-cut quartz crystals. The shift of resonance frequency of the QCM during a special washing protocol allowed us to differentiate between physically adsorbed PM fragments and any PM attached to the gold surface via chemical bonds. By washing with organic solvents, complete desorption of the wild-type PM was achieved, whereas for the SH-mutant, approximately 60\% of the PM fragments could not be detached from the surface. These PM sheets should be oriented with the cytoplasmic side facing the water subphase to that their SH-groups can chemically bind to the gold surface of the quartz plate.}, author = {Lu, T. and Rothe, U. and Liebau, M. and Schaffer, H. and Hufnagel, P. and Bakowsky, U.}, citeulike-article-id = {6097990}, citeulike-linkout-0 = {http://dx.doi.org/10.1080/713803549}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/10637773}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=10637773}, doi = {10.1080/713803549}, issn = {1521-6543}, journal = {IUBMB life}, keywords = {bacteriorhodopsin, qcm}, month = {November}, number = {5}, pages = {549--556}, posted-at = {2009-11-11 12:30:40}, priority = {4}, title = {Investigation of the orientation of purple membrane sheets in Langmuir-Blodgett films by a quartz crystal microbalance.}, url = {http://dx.doi.org/10.1080/713803549}, volume = {48}, year = {1999} }

TY - JOUR ID - Lu1999 L3 - citeulike-article-id:6097990 N2 - By means of the quartz crystal microbalance (QCM), a convenient method was developed to determine the degree of orientation of purple membrane (PM) sheets on the air/water interface. Langmuir-Blodgett films from both wild-type and SH-mutant PM (bR D36C) were vertically deposited on the surface of gold-sputtered AT-cut quartz crystals. The shift of resonance frequency of the QCM during a special washing protocol allowed us to differentiate between physically adsorbed PM fragments and any PM attached to the gold surface via chemical bonds. By washing with organic solvents, complete desorption of the wild-type PM was achieved, whereas for the SH-mutant, approximately 60% of the PM fragments could not be detached from the surface. These PM sheets should be oriented with the cytoplasmic side facing the water subphase to that their SH-groups can chemically bind to the gold surface of the quartz plate. IS - 5 JF - IUBMB life SN - 1521-6543 EP - 556 TI - Investigation of the orientation of purple membrane sheets in Langmuir-Blodgett films by a quartz crystal microbalance. VL - 48 SP - 549 KW - bacteriorhodopsin KW - qcm AU - Lu, T AU - Rothe, U AU - Liebau, M AU - Schaffer, H AU - Hufnagel, P AU - Bakowsky, U PY - 1999/11// UR - http://dx.doi.org/10.1080/713803549 ER -