Bacteriorhodopsin conjugates as anchors for supported membranes. Export

@article{Sharma2004, abstract = {The sophistication of supported lipid bilayer membranes has increased steadily as new applications are being explored. In general, tethered lipids are used to anchor the lipid bilayer to the substrate. Here we describe a new type of anchoring system for supported lipid bilayers that is based on biotin-PEG3400-bacteriorhodopsin conjugates. Amine-based coupling was used to construct the polymer conjugates, followed by fluorophore labeling to enable confocal imaging. The bacteriorhodopsin-based anchoring system was used to construct solid-supported vesicles from streptavidin-coated microspheres. This method could provide a new route for the stability enhancement of supported lipid bilayer membrane assemblies.}, author = {Sharma, Manoj K. and Jattani, Harsha and Gilchrist, M. Lane}, citeulike-article-id = {6101790}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/bc034231h}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/bc034231h}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/15264886}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=15264886}, doi = {10.1021/bc034231h}, issn = {1043-1802}, journal = {Bioconjugate chemistry}, keywords = {bacteriorhodopsin, fluorescence, membranes, vesicles}, number = {4}, pages = {942--947}, posted-at = {2009-11-12 12:00:58}, priority = {2}, title = {Bacteriorhodopsin conjugates as anchors for supported membranes.}, url = {http://dx.doi.org/10.1021/bc034231h}, volume = {15}, year = {2004} }

TY - JOUR ID - Sharma2004 L3 - citeulike-article-id:6101790 N2 - The sophistication of supported lipid bilayer membranes has increased steadily as new applications are being explored. In general, tethered lipids are used to anchor the lipid bilayer to the substrate. Here we describe a new type of anchoring system for supported lipid bilayers that is based on biotin-PEG3400-bacteriorhodopsin conjugates. Amine-based coupling was used to construct the polymer conjugates, followed by fluorophore labeling to enable confocal imaging. The bacteriorhodopsin-based anchoring system was used to construct solid-supported vesicles from streptavidin-coated microspheres. This method could provide a new route for the stability enhancement of supported lipid bilayer membrane assemblies. IS - 4 JF - Bioconjugate chemistry SN - 1043-1802 EP - 947 TI - Bacteriorhodopsin conjugates as anchors for supported membranes. VL - 15 SP - 942 KW - bacteriorhodopsin KW - fluorescence KW - membranes KW - vesicles AU - Sharma, Manoj AU - Jattani, Harsha AU - Gilchrist, Lane PY - 2004///g UR - http://dx.doi.org/10.1021/bc034231h ER -