Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity. Export

@article{citeulike:4219198, abstract = {The ability of HIV to establish latent infection in CD4+ lymphocytes represents a major barrier to the eradication of HIV. It is not clear what mechanisms favor latent over productive infection, but prior studies have suggested a role for the viral transcription factor Tat or its RNA target, TAR. Using samples from five individuals who were started on ART within 6 months of infection and achieved a viral load <50 (suppressed), we isolated one- and two-exon tat RNA from HIV propagated ex vivo from baseline plasma and from co-cultures of CD4+ T cells obtained at baseline and suppressed time points. Compared to virus from the baseline plasma (mostly from productively-infected CD4+ T cells), virus from the baseline and suppressed co-cultures (mostly from latently-infected cells) had more Tat variants with impaired transactivation activity. These findings suggest that impaired activity in the Tat-TAR axis may contribute to the establishment of latent infection in CD4+ T cells.}, author = {Yukl, Steven and Pillai, Satish and Li, Peilin and Chang, Karen and Pasutti, William and Ahlgren, Chris and Havlir, Diane and Strain, Matthew and Guenthard, Huldrych and Richman, Douglas and Rice, Andrew P P. and Daar, Eric and Little, Susan and Wong, Joseph K K.}, citeulike-article-id = {4219198}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.virol.2009.01.013}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19268337}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19268337}, day = {4}, doi = {10.1016/j.virol.2009.01.013}, issn = {1096-0341}, journal = {Virology}, keywords = {tat}, month = {March}, posted-at = {2009-03-25 13:02:08}, priority = {2}, title = {Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity.}, url = {http://dx.doi.org/10.1016/j.virol.2009.01.013}, year = {2009} }

TY - JOUR ID - citeulike:4219198 L3 - citeulike-article-id:4219198 N2 - The ability of HIV to establish latent infection in CD4+ lymphocytes represents a major barrier to the eradication of HIV. It is not clear what mechanisms favor latent over productive infection, but prior studies have suggested a role for the viral transcription factor Tat or its RNA target, TAR. Using samples from five individuals who were started on ART within 6 months of infection and achieved a viral load <50 (suppressed), we isolated one- and two-exon tat RNA from HIV propagated ex vivo from baseline plasma and from co-cultures of CD4+ T cells obtained at baseline and suppressed time points. Compared to virus from the baseline plasma (mostly from productively-infected CD4+ T cells), virus from the baseline and suppressed co-cultures (mostly from latently-infected cells) had more Tat variants with impaired transactivation activity. These findings suggest that impaired activity in the Tat-TAR axis may contribute to the establishment of latent infection in CD4+ T cells. JF - Virology SN - 1096-0341 TI - Latently-infected CD4+ T cells are enriched for HIV-1 Tat variants with impaired transactivation activity. KW - tat AU - Yukl, Steven AU - Pillai, Satish AU - Li, Peilin AU - Chang, Karen AU - Pasutti, William AU - Ahlgren, Chris AU - Havlir, Diane AU - Strain, Matthew AU - Guenthard, Huldrych AU - Richman, Douglas AU - Rice, Andrew P AU - Daar, Eric AU - Little, Susan AU - Wong, Joseph K PY - 2009/03/04/ UR - http://dx.doi.org/10.1016/j.virol.2009.01.013 ER -