Structure and real-time monitoring of the enzymatic reaction of APOBEC3G which is involved in anti-HIV activity. Export

@article{citeulike:5788605, abstract = {Human apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3G (APOBEC3G) is known to play a role in intrinsic cellular immunity against human immunodeficiency virus type 1 (HIV-1). The antiretroviral activity of APOBEC3G is associated with hypermutation of viral DNA through cytidine deamination. APOBEC3G contains two cytidine deaminase domains that are characterized by a highly conserved zinc-coordinating motif. It is known that only the C-terminal domain of APOBEC3G (c-APOBEC3G) is involved in the catalytic activity. Here, we present the solution structure and the interaction with single-stranded DNA of c-APOBEC3G. Furthermore, we have succeeded for the first time in monitoring the deamination reaction of c-APOBEC3G in real-time using NMR signals. The monitoring has demonstrated that the deamination reaction occurs in a strict 3'-->5'}, author = {Furukawa, Ayako and Nagata, Takashi and Matsugami, Akimasa and Habu, Yuichirou and Sugiyama, Ryuichi and Hayashi, Fumiaki and Kobayashi, Naohiro and Yokoyama, Shigeyuki and Takaku, Hiroshi and Katahira, Masato}, citeulike-article-id = {5788605}, citeulike-linkout-0 = {http://dx.doi.org/10.1093/nass/nrp044}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19749273}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19749273}, doi = {10.1093/nass/nrp044}, issn = {1746-8272}, journal = {Nucleic acids symposium series (2004)}, keywords = {apobec, structure}, number = {53}, pages = {87--88}, posted-at = {2009-09-15 17:43:41}, priority = {2}, title = {Structure and real-time monitoring of the enzymatic reaction of APOBEC3G which is involved in anti-HIV activity.}, url = {http://dx.doi.org/10.1093/nass/nrp044}, year = {2009} }

TY - JOUR ID - citeulike:5788605 L3 - citeulike-article-id:5788605 N2 - Human apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3G (APOBEC3G) is known to play a role in intrinsic cellular immunity against human immunodeficiency virus type 1 (HIV-1). The antiretroviral activity of APOBEC3G is associated with hypermutation of viral DNA through cytidine deamination. APOBEC3G contains two cytidine deaminase domains that are characterized by a highly conserved zinc-coordinating motif. It is known that only the C-terminal domain of APOBEC3G (c-APOBEC3G) is involved in the catalytic activity. Here, we present the solution structure and the interaction with single-stranded DNA of c-APOBEC3G. Furthermore, we have succeeded for the first time in monitoring the deamination reaction of c-APOBEC3G in real-time using NMR signals. The monitoring has demonstrated that the deamination reaction occurs in a strict 3'-->5' IS - 53 JF - Nucleic acids symposium series (2004) SN - 1746-8272 EP - 88 TI - Structure and real-time monitoring of the enzymatic reaction of APOBEC3G which is involved in anti-HIV activity. SP - 87 KW - apobec KW - structure AU - Furukawa, Ayako AU - Nagata, Takashi AU - Matsugami, Akimasa AU - Habu, Yuichirou AU - Sugiyama, Ryuichi AU - Hayashi, Fumiaki AU - Kobayashi, Naohiro AU - Yokoyama, Shigeyuki AU - Takaku, Hiroshi AU - Katahira, Masato PY - 2009/// UR - http://dx.doi.org/10.1093/nass/nrp044 ER -