Denitrifying bacteria anaerobically oxidize methane in the absence of Archaea Export

@article{citeulike:3244212, abstract = {Recently, a microbial consortium was shown to couple the anaerobic oxidation of methane to denitrification, predominantly in the form of nitrite reduction to dinitrogen gas. This consortium was dominated by bacteria of an as yet uncharacterized division and archaea of the order Methanosarcinales. The present manuscript reports on the upscaling of the enrichment culture, and addresses the role of the archaea in methane oxidation. The key gene of methanotrophic and methanogenic archaea, mcrA, was sequenced. The associated cofactor F430 was shown to have a mass of 905 Da, the same as for methanogens and different from the heavier form (951 Da) found in methanotrophic archaea. After prolonged enrichment (> 1 year), no inhibition of anaerobic methane oxidation was observed in the presence of 20 mM bromoethane sulfonate, a specific inhibitor of MCR. Optimization of the cultivation conditions led to higher rates of methane oxidation and to the decline of the archaeal population, as shown by fluorescence in situ hybridization and quantitative MALDI-TOF analysis of F430. Mass balancing showed that methane oxidation was still coupled to nitrite reduction in the total absence of oxygen. Together, our results show that bacteria can couple the anaerobic oxidation of methane to denitrification without the involvement of Archaea.}, address = {Department of Microbiology, Institute for Water and Wetland Research, Radboud University Nijmegen, NL-6525 ED Nijmegen, The Netherlands.; Department of Biochemistry, Max Planck Institute for Terrestrial Microbiology, D-35043 Marburg, Germany.}, author = {Ettwig, Katharina F. and Shima, Seigo and van de Pas-Schoonen, Katinka T. and Kahnt, J\"{o}rg and Medema, Marnix H. and den Camp, Huub J. M. and Jetten, Mike S. M. and Strous, Marc}, citeulike-article-id = {3244212}, citeulike-linkout-0 = {http://dx.doi.org/10.1111/j.1462-2920.2008.01724.x}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18721142}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18721142}, citeulike-linkout-3 = {http://www3.interscience.wiley.com/cgi-bin/abstract/121384143/ABSTRACT}, day = {20}, doi = {10.1111/j.1462-2920.2008.01724.x}, issn = {1462-2920}, journal = {Environmental Microbiology}, keywords = {anme, denitrification, physiology}, month = {August}, number = {11}, pages = {3164--3173}, posted-at = {2009-08-09 11:11:12}, priority = {0}, title = {Denitrifying bacteria anaerobically oxidize methane in the absence of Archaea}, url = {http://dx.doi.org/10.1111/j.1462-2920.2008.01724.x}, volume = {10}, year = {2008} }

TY - JOUR ID - citeulike:3244212 L3 - citeulike-article-id:3244212 N2 - Recently, a microbial consortium was shown to couple the anaerobic oxidation of methane to denitrification, predominantly in the form of nitrite reduction to dinitrogen gas. This consortium was dominated by bacteria of an as yet uncharacterized division and archaea of the order Methanosarcinales. The present manuscript reports on the upscaling of the enrichment culture, and addresses the role of the archaea in methane oxidation. The key gene of methanotrophic and methanogenic archaea, mcrA, was sequenced. The associated cofactor F430 was shown to have a mass of 905 Da, the same as for methanogens and different from the heavier form (951 Da) found in methanotrophic archaea. After prolonged enrichment (> 1 year), no inhibition of anaerobic methane oxidation was observed in the presence of 20 mM bromoethane sulfonate, a specific inhibitor of MCR. Optimization of the cultivation conditions led to higher rates of methane oxidation and to the decline of the archaeal population, as shown by fluorescence in situ hybridization and quantitative MALDI-TOF analysis of F430. Mass balancing showed that methane oxidation was still coupled to nitrite reduction in the total absence of oxygen. Together, our results show that bacteria can couple the anaerobic oxidation of methane to denitrification without the involvement of Archaea. IS - 11 JF - Environmental Microbiology SN - 1462-2920 AD - Department of Microbiology, Institute for Water and Wetland Research, Radboud University Nijmegen, NL-6525 ED Nijmegen, The Netherlands.; Department of Biochemistry, Max Planck Institute for Terrestrial Microbiology, D-35043 Marburg, Germany. EP - 3173 TI - Denitrifying bacteria anaerobically oxidize methane in the absence of Archaea VL - 10 SP - 3164 KW - anme KW - denitrification KW - physiology AU - Ettwig, Katharina AU - Shima, Seigo AU - van de Pas-Schoonen, Katinka AU - Kahnt, Jörg AU - Medema, Marnix AU - den Camp, Huub AU - Jetten, Mike AU - Strous, Marc PY - 2008/08/20/ UR - http://dx.doi.org/10.1111/j.1462-2920.2008.01724.x ER -