Role of NRSF/REST in the molecular mechanisms regulating neural-specific expression of trkC/neurotrophin-3 receptor gene. Export

@article{citeulike:1533949, abstract = {The processes of differentiation and development of neurons involve the induction of neuron-specific genes by instructive signals with subsequent neurotrophic factor-driven survival and functional maturation. We have previously shown that bone morphogenetic protein-2 (BMP2) and retinoic acid synergistically induce the responsiveness of developing sympathetic neurons to neurotrophic factors, neurotrophin 3 (NT-3), and GDNF by upregulating corresponding receptors concomitantly with the induction of other neuron-specific genes including BRINP1, a neuron-specific cell-cycle regulatory protein. In the present study, we analyzed transcriptional mechanisms regulating the neuron-specific expression of TrkC/NT-3 receptor gene. TrkC gene contains at least four NRSE/RE-1 (neuron-restrictive silencing element/repressor element 1)-like elements (TrkC-NRSE A-D). Consequently, we found that in non-neuronal cells, neuron-restrictive silencing factor (NRSF) acts on TrkC-NRSE D located at the downstream of exon 3 to suppress the promoter activity of TrkC gene in a manner similar to the mechanism of NRSF suppressing BRINP1 transcription. In contrast, in neuronal cells, the biological activity of NRSF on TrkC was suppressed. From these observations, molecular mechanisms regulating the expression of neuron-specific genes via NRSE during neuronal differentiation are discussed.}, address = {Division of Innovative Research, Creative Research Initiative "Sousei" (CRIS), Hokkaido University, Kita-21-Nishi-10, Kita-ku, Sapporo 001-0021, Japan.}, author = {Nakatani, T. and Ueno, S. and Mori, N. and Matsuoka, I.}, citeulike-article-id = {1533949}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.molbrainres.2004.12.019}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/15857687}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=15857687}, day = {27}, doi = {10.1016/j.molbrainres.2004.12.019}, issn = {0169-328X}, journal = {Brain Res Mol Brain Res}, keywords = {05, expression, gene\_, rest, trkc-nt-3}, month = {April}, number = {1-2}, pages = {249--259}, posted-at = {2008-09-11 13:32:12}, priority = {2}, title = {Role of NRSF/REST in the molecular mechanisms regulating neural-specific expression of trkC/neurotrophin-3 receptor gene.}, url = {http://dx.doi.org/10.1016/j.molbrainres.2004.12.019}, volume = {135}, year = {2005} }

TY - JOUR ID - citeulike:1533949 L3 - citeulike-article-id:1533949 N2 - The processes of differentiation and development of neurons involve the induction of neuron-specific genes by instructive signals with subsequent neurotrophic factor-driven survival and functional maturation. We have previously shown that bone morphogenetic protein-2 (BMP2) and retinoic acid synergistically induce the responsiveness of developing sympathetic neurons to neurotrophic factors, neurotrophin 3 (NT-3), and GDNF by upregulating corresponding receptors concomitantly with the induction of other neuron-specific genes including BRINP1, a neuron-specific cell-cycle regulatory protein. In the present study, we analyzed transcriptional mechanisms regulating the neuron-specific expression of TrkC/NT-3 receptor gene. TrkC gene contains at least four NRSE/RE-1 (neuron-restrictive silencing element/repressor element 1)-like elements (TrkC-NRSE A-D). Consequently, we found that in non-neuronal cells, neuron-restrictive silencing factor (NRSF) acts on TrkC-NRSE D located at the downstream of exon 3 to suppress the promoter activity of TrkC gene in a manner similar to the mechanism of NRSF suppressing BRINP1 transcription. In contrast, in neuronal cells, the biological activity of NRSF on TrkC was suppressed. From these observations, molecular mechanisms regulating the expression of neuron-specific genes via NRSE during neuronal differentiation are discussed. IS - 1-2 JF - Brain Res Mol Brain Res SN - 0169-328X AD - Division of Innovative Research, Creative Research Initiative "Sousei" (CRIS), Hokkaido University, Kita-21-Nishi-10, Kita-ku, Sapporo 001-0021, Japan. EP - 259 TI - Role of NRSF/REST in the molecular mechanisms regulating neural-specific expression of trkC/neurotrophin-3 receptor gene. VL - 135 SP - 249 KW - 05 KW - expression KW - gene_ KW - rest KW - trkc-nt-3 AU - Nakatani, T AU - Ueno, S AU - Mori, N AU - Matsuoka, I PY - 2005/04/27/ UR - http://dx.doi.org/10.1016/j.molbrainres.2004.12.019 ER -