The cryoloop: an adaptable reversible cooling deactivation method for behavioral or electrophysiological assessment of neural function. Export

@article{citeulike:5998093, abstract = {We describe a very adaptable reversible inactivation technique for the behavioral or electrophysiological analysis of neural circuits. The cryoloop device can be permanently implanted or topically applied in an acute preparation to apply cold to discrete surface regions of the central nervous system (e.g. cerebral cortex or midbrain). The cryoloop consists of a custom shaped, stainless steel, hypodermic tubing and cooling is effected by passing chilled methanol through the lumen of the tubing. Cryoloop temperature is monitored by a microthermocouple attached to the union of the loop, and can be maintained within +/- 1 degrees C of a desired temperature. In chronic preparations, implanted cryoloops have been maintained in cats and monkeys for periods in excess of 2 years. After this period there are no structural, metabolic of functional changes in the deactivated tissue, and full reversibility of cooling-induced effects is maintained. Operation of multiple cryoprobes provides great flexibility of experimental protocols, permits double and triple functional dissociations to be made, and strengthens experimental design considerably.}, author = {Lomber, S. G. and Payne, B. R. and Horel, J. A.}, citeulike-article-id = {5998093}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/10065985}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=10065985}, issn = {0165-0270}, journal = {Journal of neuroscience methods}, keywords = {cooling, inactivation, technique}, month = {January}, number = {2}, pages = {179--194}, posted-at = {2009-10-23 16:21:16}, priority = {2}, title = {The cryoloop: an adaptable reversible cooling deactivation method for behavioral or electrophysiological assessment of neural function.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/10065985}, volume = {86}, year = {1999} }

TY - JOUR ID - citeulike:5998093 L3 - citeulike-article-id:5998093 N2 - We describe a very adaptable reversible inactivation technique for the behavioral or electrophysiological analysis of neural circuits. The cryoloop device can be permanently implanted or topically applied in an acute preparation to apply cold to discrete surface regions of the central nervous system (e.g. cerebral cortex or midbrain). The cryoloop consists of a custom shaped, stainless steel, hypodermic tubing and cooling is effected by passing chilled methanol through the lumen of the tubing. Cryoloop temperature is monitored by a microthermocouple attached to the union of the loop, and can be maintained within +/- 1 degrees C of a desired temperature. In chronic preparations, implanted cryoloops have been maintained in cats and monkeys for periods in excess of 2 years. After this period there are no structural, metabolic of functional changes in the deactivated tissue, and full reversibility of cooling-induced effects is maintained. Operation of multiple cryoprobes provides great flexibility of experimental protocols, permits double and triple functional dissociations to be made, and strengthens experimental design considerably. IS - 2 JF - Journal of neuroscience methods SN - 0165-0270 EP - 194 TI - The cryoloop: an adaptable reversible cooling deactivation method for behavioral or electrophysiological assessment of neural function. VL - 86 SP - 179 KW - cooling KW - inactivation KW - technique AU - Lomber, SG AU - Payne, BR AU - Horel, JA PY - 1999/01// UR - http://view.ncbi.nlm.nih.gov/pubmed/10065985 ER -