Global survey of organ and organelle protein expression in mouse: combined proteomic and transcriptomic profiling. Export

@article{citeulike:2231193, abstract = {Organs and organelles represent core biological systems in mammals, but the diversity in protein composition remains unclear. Here, we combine subcellular fractionation with exhaustive tandem mass spectrometry-based shotgun sequencing to examine the protein content of four major organellar compartments (cytosol, membranes [microsomes], mitochondria, and nuclei) in six organs (brain, heart, kidney, liver, lung, and placenta) of the laboratory mouse, Mus musculus. Using rigorous statistical filtering and machine-learning methods, the subcellular localization of 3274 of the 4768 proteins identified was determined with high confidence, including 1503 previously uncharacterized factors, while tissue selectivity was evaluated by comparison to previously reported mRNA expression patterns. This molecular compendium, fully accessible via a searchable web-browser interface, serves as a reliable reference of the expressed tissue and organelle proteomes of a leading model mammal.}, address = {Banting and Best Department of Medical Research, University of Toronto, Toronto, ON M5G 1L6, Canada.}, author = {Kislinger, T. and Cox, B. and Kannan, A. and Chung, C. and Hu, P. and Ignatchenko, A. and Scott, M. S. and Gramolini, A. O. and Morris, Q. and Hallett, M. T. and Rossant, J. and Hughes, T. R. and Frey, B. and Emili, A.}, citeulike-article-id = {2231193}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.cell.2006.01.044}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16615898}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16615898}, day = {7}, doi = {10.1016/j.cell.2006.01.044}, issn = {0092-8674}, journal = {Cell}, keywords = {mice, organelle, protein}, month = {April}, number = {1}, pages = {173--186}, posted-at = {2008-01-14 17:26:26}, priority = {0}, title = {Global survey of organ and organelle protein expression in mouse: combined proteomic and transcriptomic profiling.}, url = {http://dx.doi.org/10.1016/j.cell.2006.01.044}, volume = {125}, year = {2006} }

TY - JOUR ID - citeulike:2231193 L3 - citeulike-article-id:2231193 N2 - Organs and organelles represent core biological systems in mammals, but the diversity in protein composition remains unclear. Here, we combine subcellular fractionation with exhaustive tandem mass spectrometry-based shotgun sequencing to examine the protein content of four major organellar compartments (cytosol, membranes [microsomes], mitochondria, and nuclei) in six organs (brain, heart, kidney, liver, lung, and placenta) of the laboratory mouse, Mus musculus. Using rigorous statistical filtering and machine-learning methods, the subcellular localization of 3274 of the 4768 proteins identified was determined with high confidence, including 1503 previously uncharacterized factors, while tissue selectivity was evaluated by comparison to previously reported mRNA expression patterns. This molecular compendium, fully accessible via a searchable web-browser interface, serves as a reliable reference of the expressed tissue and organelle proteomes of a leading model mammal. IS - 1 JF - Cell SN - 0092-8674 AD - Banting and Best Department of Medical Research, University of Toronto, Toronto, ON M5G 1L6, Canada. EP - 186 TI - Global survey of organ and organelle protein expression in mouse: combined proteomic and transcriptomic profiling. VL - 125 SP - 173 KW - mice KW - organelle KW - protein AU - Kislinger, T AU - Cox, B AU - Kannan, A AU - Chung, C AU - Hu, P AU - Ignatchenko, A AU - Scott, MS AU - Gramolini, AO AU - Morris, Q AU - Hallett, MT AU - Rossant, J AU - Hughes, TR AU - Frey, B AU - Emili, A PY - 2006/04/07/ UR - http://dx.doi.org/10.1016/j.cell.2006.01.044 ER -