Investigation of the immobilisation of bovine serum albumin, trypsin, acid phosphatase and alkaline phosphatase to poly(hydroxyethyl acrylate)-co-cellulose and poly(hydroxyethyl acrylate)-co-pectin

Poly(hydroxyethyl methacrylate)-co-pectins (107% graft), activated with p-benzoquinone, immobilised 122 mg of bovine serum albumin (BSA) per g of copolymer and 22.4 mg of trypsin per g of copolymer. This latter figure was replicated when p-toluene sulphonyl chloride was used for activating the copolymer. In a separate exercise p-benzoquinone was used to couple diaminohexane to the copolymer. This modified product immobilsed 396 mg of trypsin/g when glutaraldehyde was used in coupling. A 25% grafted, poly(hydroxyethyl methacrylate)-co-cellulose immobilised 39.5 mg of BSA per g of copolymer.