Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy. Export

@article{citeulike:3397003, abstract = {A long-standing goal of biology is to map the behavior of all cells during vertebrate embryogenesis. We developed digital scanned laser light sheet fluorescence microscopy and recorded nuclei localization and movement in entire wild-type and mutant zebrafish embryos over the first 24 hours of development. Multiview in vivo imaging at 1.5 billion voxels per minute provides "digital embryos" (i.e., comprehensive databases of cell positions, divisions, and migratory tracks). Our analysis of global cell division patterns reveals a maternally defined initial morphodynamic symmetry break, which identifies the embryonic body axis. We further derive a model of germ layer formation and show that the mesendoderm forms from one-third of the embryo's cells in a single event. Our digital embryos, with 55 million nucleus entries, are provided as a resource.}, author = {Keller, Philipp J J. and Schmidt, Annette D D. and Wittbrodt, Joachim and Stelzer, Ernst H K H.}, citeulike-article-id = {3397003}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1162493}, citeulike-linkout-1 = {http://www.sciencemag.org/cgi/content/abstract/322/5904/1065}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18845710}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18845710}, day = {9}, doi = {10.1126/science.1162493}, issn = {1095-9203}, journal = {Science (New York, N.Y.)}, keywords = {embryo}, month = {October}, posted-at = {2008-10-15 14:10:05}, priority = {2}, title = {Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy.}, url = {http://dx.doi.org/10.1126/science.1162493}, year = {2008} }

TY - JOUR ID - citeulike:3397003 L3 - citeulike-article-id:3397003 N2 - A long-standing goal of biology is to map the behavior of all cells during vertebrate embryogenesis. We developed digital scanned laser light sheet fluorescence microscopy and recorded nuclei localization and movement in entire wild-type and mutant zebrafish embryos over the first 24 hours of development. Multiview in vivo imaging at 1.5 billion voxels per minute provides "digital embryos" (i.e., comprehensive databases of cell positions, divisions, and migratory tracks). Our analysis of global cell division patterns reveals a maternally defined initial morphodynamic symmetry break, which identifies the embryonic body axis. We further derive a model of germ layer formation and show that the mesendoderm forms from one-third of the embryo's cells in a single event. Our digital embryos, with 55 million nucleus entries, are provided as a resource. JF - Science (New York, N.Y.) SN - 1095-9203 TI - Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy. KW - embryo AU - Keller, Philipp J AU - Schmidt, Annette D AU - Wittbrodt, Joachim AU - Stelzer, Ernst H K PY - 2008/10/09/ UR - http://dx.doi.org/10.1126/science.1162493 ER -