Regulation of Microtubule Dynamics by Reaction Cascades Around Chromosomes

@article{citeulike:3445930, abstract = {During spindle assembly, chromosomes generate gradients of microtubule stabilization through a reaction-diffusion process, but how this is achieved is not well understood. We have measured the spatial distribution of microtubule aster asymmetry around chromosomes by incubating centrosomes and micro-patterned chromatin patches in frog egg extracts. We then screened for microtubule stabilization gradient shapes that would generate such spatial distributions using computer simulations. Only a long-range sharply decaying microtubule stabilization gradient could generate aster asymmetries fitting the experimental data. We propose a reaction diffusion model that combines the chromosome generated Ran-GTP-Importin reaction network to a secondary phosphorylation network as a potential mechanism for the generation of such gradients. 10.1126/science.1161820}, author = {Athale, Chaitanya A. and Dinarina, Ana and Mora-Coral, Maria and Pugieux, Celine and Nedelec, Francois and Karsenti, Eric}, citeulike-article-id = {3445930}, comment = {Check it}, doi = {10.1126/science.1161820}, journal = {Science}, keywords = {microtubules}, month = {October}, pages = {1161820+}, posted-at = {2008-10-24 08:55:41}, priority = {2}, title = {Regulation of Microtubule Dynamics by Reaction Cascades Around Chromosomes}, url = {http://dx.doi.org/10.1126/science.1161820}, year = {2008} }

TY - JOUR ID - citeulike:3445930 L3 - citeulike-article-id:3445930 N2 - During spindle assembly, chromosomes generate gradients of microtubule stabilization through a reaction-diffusion process, but how this is achieved is not well understood. We have measured the spatial distribution of microtubule aster asymmetry around chromosomes by incubating centrosomes and micro-patterned chromatin patches in frog egg extracts. We then screened for microtubule stabilization gradient shapes that would generate such spatial distributions using computer simulations. Only a long-range sharply decaying microtubule stabilization gradient could generate aster asymmetries fitting the experimental data. We propose a reaction diffusion model that combines the chromosome generated Ran-GTP-Importin reaction network to a secondary phosphorylation network as a potential mechanism for the generation of such gradients. 10.1126/science.1161820 JF - Science TI - Regulation of Microtubule Dynamics by Reaction Cascades Around Chromosomes SP - 1161820 KW - microtubules N1 - Check it AU - Athale, Chaitanya AU - Dinarina, Ana AU - Mora-Coral, Maria AU - Pugieux, Celine AU - Nedelec, Francois AU - Karsenti, Eric PY - 2008/10/23/ UR - http://dx.doi.org/10.1126/science.1161820 ER -