Nucleotide pools of growing, synchronized and stressed cultures of Saccharomyces cerevisiae Export

@article{Dit_etal83, abstract = {High pressure liquid chromatography has been used to study the acid soluble nucleotide pool of Saccharomyces cerevisiae under different conditions of growth. ATP, ADP, AMP, NAD, GTP, UTP, UDP, CTP, CDP, and UDP-sugars plus UMP could be separated and were found in concentrations higher than 0.1 mumol per g yeast cell dry weight (= detection limit). During glucose-limited continuous culture the levels of individual nucleotides depended on the growth rate, which was most pronounced with pyrimidine (uridine, cytidine) nucleotides. The energy charge (E.C.) remained high (0.9) at all growth rates (0.07-0.3 h-1). During synchronized growth at a constant growth rate (0.11 h-1) almost all nucleotide levels and the E.C. remained at constant values with the only exception of UDP-sugars and UMP of which increased levels were found during the phase of budding. Under conditions of metabolic stress (addition of antimycin A, deoxyglucose plus iodoacetate) pronounced changes in the levels of purine (adenine and guanine) nucleotides and the E.C. were observed. All other nucleotides were less influenced by these conditions. Only under these conditions IMP accumulation was observed. The results strongly argue against the significance of purine nucleotide or E.C. measurements under viable conditions. In contrast, changes in the levels of pyrimidine nucleotides seem to be indicative of changes in the flux through the metabolic pathways where they act as coenzymes.}, author = {Ditzelm{\"u}ller, G. and W{\"o}hrer, W. and Kubicek, C. P. and R{\"o}hr, M.}, citeulike-article-id = {1700564}, journal = {Arch Microbiol}, key = {D35}, keywords = {6354131, 99m, abstract, aged, agents, animals, arrhythmia, artificial, bias, cardiac, cardiography, cerevisiae, chromatography, comparative, compounds, congestive, contraction, digitalis, digitoxin, diphosphates, diuretics, dogs, electrodes, english, epidemiology, failure, female, gluconates, glucose, glycosides, heart, high, humans, hyperkalemia, impedance, liquid, male, mathematics, middle, myocardial, nucleotides, of, organotechnetium, output, pericarditis, pressure, procedures, prospective, pyrophosphate, rate, reproducibility, respiration, results, saccharomyces, stroke, studies, study, surgical, tc, technetium, thermodilution, vasodilator, volume}, month = {Aug}, number = {1}, pages = {63--67}, posted-at = {2007-09-27 15:19:49}, priority = {2}, title = {Nucleotide pools of growing, synchronized and stressed cultures of \emph{{S}accharomyces cerevisiae}}, volume = {135}, year = {1983} }

TY - JOUR ID - Dit_etal83 L3 - citeulike-article-id:1700564 N2 - High pressure liquid chromatography has been used to study the acid soluble nucleotide pool of Saccharomyces cerevisiae under different conditions of growth. ATP, ADP, AMP, NAD, GTP, UTP, UDP, CTP, CDP, and UDP-sugars plus UMP could be separated and were found in concentrations higher than 0.1 mumol per g yeast cell dry weight (= detection limit). During glucose-limited continuous culture the levels of individual nucleotides depended on the growth rate, which was most pronounced with pyrimidine (uridine, cytidine) nucleotides. The energy charge (E.C.) remained high (0.9) at all growth rates (0.07-0.3 h-1). During synchronized growth at a constant growth rate (0.11 h-1) almost all nucleotide levels and the E.C. remained at constant values with the only exception of UDP-sugars and UMP of which increased levels were found during the phase of budding. Under conditions of metabolic stress (addition of antimycin A, deoxyglucose plus iodoacetate) pronounced changes in the levels of purine (adenine and guanine) nucleotides and the E.C. were observed. All other nucleotides were less influenced by these conditions. Only under these conditions IMP accumulation was observed. The results strongly argue against the significance of purine nucleotide or E.C. measurements under viable conditions. In contrast, changes in the levels of pyrimidine nucleotides seem to be indicative of changes in the flux through the metabolic pathways where they act as coenzymes. IS - 1 JF - Arch Microbiol EP - 67 TI - Nucleotide pools of growing, synchronized and stressed cultures of \emph{{S}accharomyces cerevisiae} VL - 135 SP - 63 KW - 6354131 KW - 99m KW - abstract KW - aged KW - agents KW - animals KW - arrhythmia KW - artificial KW - bias KW - cardiac KW - cardiography KW - cerevisiae KW - chromatography KW - comparative KW - compounds KW - congestive KW - contraction KW - digitalis KW - digitoxin KW - diphosphates KW - diuretics KW - dogs KW - electrodes KW - english KW - epidemiology KW - failure KW - female KW - gluconates KW - glucose KW - glycosides KW - heart KW - high KW - humans KW - hyperkalemia KW - impedance KW - liquid KW - male KW - mathematics KW - middle KW - myocardial KW - nucleotides KW - of KW - organotechnetium KW - output KW - pericarditis KW - pressure KW - procedures KW - prospective KW - pyrophosphate KW - rate KW - reproducibility KW - respiration KW - results KW - saccharomyces KW - stroke KW - studies KW - study KW - surgical KW - tc KW - technetium KW - thermodilution KW - vasodilator KW - volume AU - Ditzelm{\"u}ller, G AU - W{\"o}hrer, W AU - Kubicek, CP AU - R{\"o}hr, M PY - 1983/Aug// ER -