Efficient developmental mis-targeting by the sporamin NTPP vacuolar signal to plastids in young leaves of sugarcane and Arabidopsis. Export

@article{GnaBir04, abstract = {Plant vacuoles are multi-functional, developmentally varied and can occupy up to 90\% of plant cells. The N-terminal propeptide (NTPP) of sweet potato sporamin and the C-terminal propeptide (CTPP) of tobacco chitinase have been developed as models to target some heterologous proteins to vacuoles but so far tested on only a few plant species, vacuole types and "payload" proteins. Most studies have focused on lytic and protein-storage vacuoles, which may differ substantially from the sugar-storage vacuoles in crops like sugarcane. Our results extend the evidence that NTPP of sporamin can direct heterologous proteins to vacuoles in diverse plant species and indicate that sugarcane sucrose-storage vacuoles (like the lytic vacuoles in other plant species) are hostile to heterologous proteins. A low level of cytosolic NTPP-GFP (green fluorescent protein) was detectable in most cell types in sugarcane and Arabidopsis, but only Arabidopsis mature leaf mesophyll cells accumulated NTPP-GFP to detectable levels in vacuoles. Unexpectedly, efficient developmental mis-trafficking of NTPP-GFP to chloroplasts was found in young leaf mesophyll cells of both species. Vacuolar targeting by tobacco chitinase CTPP was inefficient in sugarcane, leaving substantial cytoplasmic activity of rat lysosomal beta-glucuronidase (GUS) [ER (endoplasmic reticulum)-RGUS-CTPP]. Sporamin NTPP is a promising targeting signal for studies of vacuolar function and for metabolic engineering. Such applications must take account of the efficient developmental mis-targeting by the signal and the instability of most introduced proteins, even in "storage vacuoles".}, author = {Gnanasambandam, Annathurai and Birch, Robert G.}, citeulike-article-id = {1700682}, citeulike-linkout-0 = {http://dx.doi.org/10.1007/s00299-004-0860-5}, doi = {10.1007/s00299-004-0860-5}, journal = {Plant Cell Rep}, keywords = {15372194, 16s, abstract, agriculture, air, alignment, analysis, animals, ascomycota, base, biotechnology, brazil, cellulose, chloride, chloroplast, chromosome, comparative, complex, concentration, cuba, culture, data, dioxide, dna, electron, english, environmental, evolution, factors, fungi, genetics, genome, govt, hydrogen-ion, incineration, iv, lepidoptera, mapping, media, microbiology, mitochondrial, molecular, monitoring, mutation, nitrogen, non-us, paper, phylogeny, plant, pollutants, primers, research, ribosomal, rna, saccharum, sequence, smoke, sodium, species, specificity, spectrophotometry, study, support, temperature, time, transport, ultraviolet, variation, water, xylosidases}, month = {Sep}, posted-at = {2007-09-27 15:19:56}, priority = {2}, title = {Efficient developmental mis-targeting by the sporamin {NTPP} vacuolar signal to plastids in young leaves of sugarcane and {A}rabidopsis.}, url = {10.1007/s00299-004-0860-5}, year = {2004} }

TY - JOUR ID - GnaBir04 L3 - citeulike-article-id:1700682 N2 - Plant vacuoles are multi-functional, developmentally varied and can occupy up to 90\% of plant cells. The N-terminal propeptide (NTPP) of sweet potato sporamin and the C-terminal propeptide (CTPP) of tobacco chitinase have been developed as models to target some heterologous proteins to vacuoles but so far tested on only a few plant species, vacuole types and "payload" proteins. Most studies have focused on lytic and protein-storage vacuoles, which may differ substantially from the sugar-storage vacuoles in crops like sugarcane. Our results extend the evidence that NTPP of sporamin can direct heterologous proteins to vacuoles in diverse plant species and indicate that sugarcane sucrose-storage vacuoles (like the lytic vacuoles in other plant species) are hostile to heterologous proteins. A low level of cytosolic NTPP-GFP (green fluorescent protein) was detectable in most cell types in sugarcane and Arabidopsis, but only Arabidopsis mature leaf mesophyll cells accumulated NTPP-GFP to detectable levels in vacuoles. Unexpectedly, efficient developmental mis-trafficking of NTPP-GFP to chloroplasts was found in young leaf mesophyll cells of both species. Vacuolar targeting by tobacco chitinase CTPP was inefficient in sugarcane, leaving substantial cytoplasmic activity of rat lysosomal beta-glucuronidase (GUS) [ER (endoplasmic reticulum)-RGUS-CTPP]. Sporamin NTPP is a promising targeting signal for studies of vacuolar function and for metabolic engineering. Such applications must take account of the efficient developmental mis-targeting by the signal and the instability of most introduced proteins, even in "storage vacuoles". JF - Plant Cell Rep TI - Efficient developmental mis-targeting by the sporamin {NTPP} vacuolar signal to plastids in young leaves of sugarcane and {A}rabidopsis. KW - 15372194 KW - 16s KW - abstract KW - agriculture KW - air KW - alignment KW - analysis KW - animals KW - ascomycota KW - base KW - biotechnology KW - brazil KW - cellulose KW - chloride KW - chloroplast KW - chromosome KW - comparative KW - complex KW - concentration KW - cuba KW - culture KW - data KW - dioxide KW - dna KW - electron KW - english KW - environmental KW - evolution KW - factors KW - fungi KW - genetics KW - genome KW - govt KW - hydrogen-ion KW - incineration KW - iv KW - lepidoptera KW - mapping KW - media KW - microbiology KW - mitochondrial KW - molecular KW - monitoring KW - mutation KW - nitrogen KW - non-us KW - paper KW - phylogeny KW - plant KW - pollutants KW - primers KW - research KW - ribosomal KW - rna KW - saccharum KW - sequence KW - smoke KW - sodium KW - species KW - specificity KW - spectrophotometry KW - study KW - support KW - temperature KW - time KW - transport KW - ultraviolet KW - variation KW - water KW - xylosidases AU - Gnanasambandam, Annathurai AU - Birch, Robert PY - 2004/Sep// UR - http://dx.doi.org/10.1007/s00299-004-0860-5 ER -