Directed Neural Differentiation of Human Embryonic Stem Cells via an Obligated Primitive Anterior Stage Export

@article{citeulike:2100584, abstract = {Understanding neuroectoderm formation and subsequent diversification to functional neural subtypes remains elusive. We show here that human embryonic stem cells (hESCs) differentiate to primitive neuroectoderm after 8-10 days. At this stage, cells uniformly exhibit columnar morphology and express neural markers, including anterior but not posterior homeodomain proteins. The anterior identity of these cells develops regardless of morphogens present during initial neuroectoderm specification. This anterior phenotype can be maintained or transformed to a caudal fate with specific morphogens over the next week, when cells become definitive neuroepithelia, marked by neural tube-like structures with distinct adhesion molecule expression, Sox1 expression, and a resistance to additional patterning signals. Thus, primitive neuroepithelia represents the earliest neural cells that possess the potential to differentiate to regionally specific neural progenitors. This finding offers insights into early human brain development and lays a foundation for generating neural cells with correct positional and transmitter profiles. Disclosure of potential conflicts of interest is found at the end of this article. 10.1634/stemcells.2006-0707}, author = {Pankratz, Matthew T. and Li, Xue-Jun and Lavaute, Timothy M. and Lyons, Elizabeth A. and Chen, Xin and Zhang, Su-Chun}, citeulike-article-id = {2100584}, citeulike-linkout-0 = {http://dx.doi.org/10.1634/stemcells.2006-0707}, citeulike-linkout-1 = {http://stemcells.alphamedpress.org/cgi/content/abstract/25/6/1511}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/17332508}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=17332508}, day = {1}, doi = {10.1634/stemcells.2006-0707}, journal = {Stem Cells}, keywords = {cellules\_souches, cellules\_souches\_embryonnaires\_humaines, human\_embryonic\_stem\_cells, stem\_cells}, month = {June}, number = {6}, pages = {1511--1520}, posted-at = {2008-02-26 16:07:36}, priority = {2}, title = {Directed Neural Differentiation of Human Embryonic Stem Cells via an Obligated Primitive Anterior Stage}, url = {http://dx.doi.org/10.1634/stemcells.2006-0707}, volume = {25}, year = {2007} }

TY - JOUR ID - citeulike:2100584 L3 - citeulike-article-id:2100584 N2 - Understanding neuroectoderm formation and subsequent diversification to functional neural subtypes remains elusive. We show here that human embryonic stem cells (hESCs) differentiate to primitive neuroectoderm after 8-10 days. At this stage, cells uniformly exhibit columnar morphology and express neural markers, including anterior but not posterior homeodomain proteins. The anterior identity of these cells develops regardless of morphogens present during initial neuroectoderm specification. This anterior phenotype can be maintained or transformed to a caudal fate with specific morphogens over the next week, when cells become definitive neuroepithelia, marked by neural tube-like structures with distinct adhesion molecule expression, Sox1 expression, and a resistance to additional patterning signals. Thus, primitive neuroepithelia represents the earliest neural cells that possess the potential to differentiate to regionally specific neural progenitors. This finding offers insights into early human brain development and lays a foundation for generating neural cells with correct positional and transmitter profiles. Disclosure of potential conflicts of interest is found at the end of this article. 10.1634/stemcells.2006-0707 IS - 6 JF - Stem Cells EP - 1520 TI - Directed Neural Differentiation of Human Embryonic Stem Cells via an Obligated Primitive Anterior Stage VL - 25 SP - 1511 KW - cellules_souches KW - cellules_souches_embryonnaires_humaines KW - human_embryonic_stem_cells KW - stem_cells AU - Pankratz, Matthew AU - Li, Xue-Jun AU - Lavaute, Timothy AU - Lyons, Elizabeth AU - Chen, Xin AU - Zhang, Su-Chun PY - 2007/06/01/ UR - http://dx.doi.org/10.1634/stemcells.2006-0707 ER -