Assays and properties of the Arf GAPs AGAP1, ASAP1, and Arf GAP1. Export

@article{citeulike:1512867, abstract = {ADP-ribosylation factors (Arfs) are Ras-like GTP-binding proteins that regulate membrane traffic and actin remodeling. Arf function requires GTP hydrolysis but Arf lacks GTPase activity; consequently, Arf function is dependent on Arf GTPase-activating proteins (GAPs). The Arf GAPs are a structurally diverse group of at least 16 proteins. Several Arf GAPs use a single Arf isoform. However, due to structural differences, the conditions supporting productive interactions between Arf and different Arf GAPs vary. Here, we describe preparation and basic properties of three Arf GAPs. We use these proteins to illustrate assays for Arf GAP activity. Conditions that optimize activity for each GAP are discussed. These methods can be used for the further characterization of Arf-Arf GAP interaction that is necessary for understanding the function of Arf in cellular physiology.}, address = {Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.}, author = {Che, M. M. and Nie, Z. and Randazzo, P. A.}, citeulike-article-id = {1512867}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/S0076-6879(05)04015-2}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16413266}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16413266}, doi = {10.1016/S0076-6879(05)04015-2}, issn = {0076-6879}, journal = {Methods Enzymol}, keywords = {arf, gap}, pages = {147--163}, posted-at = {2007-07-30 14:39:09}, priority = {2}, title = {Assays and properties of the Arf GAPs AGAP1, ASAP1, and Arf GAP1.}, url = {http://dx.doi.org/10.1016/S0076-6879(05)04015-2}, volume = {404}, year = {2005} }

TY - JOUR ID - citeulike:1512867 L3 - citeulike-article-id:1512867 N2 - ADP-ribosylation factors (Arfs) are Ras-like GTP-binding proteins that regulate membrane traffic and actin remodeling. Arf function requires GTP hydrolysis but Arf lacks GTPase activity; consequently, Arf function is dependent on Arf GTPase-activating proteins (GAPs). The Arf GAPs are a structurally diverse group of at least 16 proteins. Several Arf GAPs use a single Arf isoform. However, due to structural differences, the conditions supporting productive interactions between Arf and different Arf GAPs vary. Here, we describe preparation and basic properties of three Arf GAPs. We use these proteins to illustrate assays for Arf GAP activity. Conditions that optimize activity for each GAP are discussed. These methods can be used for the further characterization of Arf-Arf GAP interaction that is necessary for understanding the function of Arf in cellular physiology. JF - Methods Enzymol SN - 0076-6879 AD - Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA. EP - 163 TI - Assays and properties of the Arf GAPs AGAP1, ASAP1, and Arf GAP1. VL - 404 SP - 147 KW - arf KW - gap AU - Che, MM AU - Nie, Z AU - Randazzo, PA PY - 2005/// UR - http://dx.doi.org/10.1016/S0076-6879(05)04015-2 ER -