Adenine nucleotide translocator isoforms 1 and 2 are differently distributed in the mitochondrial inner membrane and have distinct affinities to cyclophilin D. Export

@article{citeulike:1669130, abstract = {Different isoforms of the adenine nucleotide translocase (ANT) are expressed in a tissue-specific manner. It was assumed that ANT-1 and ANT-2 co-exist in every single mitochondrion and might be differently distributed within the membrane structures that constitute the peripheral inner membrane or the crista membrane. To discriminate between ANT originating from peripheral or from cristal inner membranes we made use of the fact that complexes between porin, the outer-membrane pore protein, and the ANT can be generated. Such complexes between porin and the ANT in the peripheral inner membrane were induced in rat heart mitochondria and isolated from rat brain and kidney. Using ANT-isotype-specific antibodies and sequence analysis of the N-terminal end, it was discovered that the peripheral inner membrane contained ANT-1 and ANT-2, whereas the cristal membrane contained exclusively ANT-2. Cyclophilin was co-purified with the porin-ANT complexes, whereas it was absent in the crista-derived ANT. This suggested that ANT-1 might have a higher affinity for cyclophilin. This specific intra-mitochondrial distribution of the two ANT isotypes and cyclophilin D suggests specific functions of the peripheral and crista-forming parts of the inner membrane and the two ANT isotypes therein.}, address = {A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, 119899, Russia.}, author = {Vyssokikh, M. Y. and Katz, A. and Rueck, A. and Wuensch, C. and D\"{o}rner, A. and Zorov, D. B. and Brdiczka, D.}, citeulike-article-id = {1669130}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/11513733}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=11513733}, day = {1}, issn = {0264-6021}, journal = {Biochem J}, keywords = {ant, mitochondria}, month = {September}, number = {Pt 2}, pages = {349--358}, posted-at = {2007-09-18 13:52:11}, priority = {2}, title = {Adenine nucleotide translocator isoforms 1 and 2 are differently distributed in the mitochondrial inner membrane and have distinct affinities to cyclophilin D.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/11513733}, volume = {358}, year = {2001} }

TY - JOUR ID - citeulike:1669130 L3 - citeulike-article-id:1669130 N2 - Different isoforms of the adenine nucleotide translocase (ANT) are expressed in a tissue-specific manner. It was assumed that ANT-1 and ANT-2 co-exist in every single mitochondrion and might be differently distributed within the membrane structures that constitute the peripheral inner membrane or the crista membrane. To discriminate between ANT originating from peripheral or from cristal inner membranes we made use of the fact that complexes between porin, the outer-membrane pore protein, and the ANT can be generated. Such complexes between porin and the ANT in the peripheral inner membrane were induced in rat heart mitochondria and isolated from rat brain and kidney. Using ANT-isotype-specific antibodies and sequence analysis of the N-terminal end, it was discovered that the peripheral inner membrane contained ANT-1 and ANT-2, whereas the cristal membrane contained exclusively ANT-2. Cyclophilin was co-purified with the porin-ANT complexes, whereas it was absent in the crista-derived ANT. This suggested that ANT-1 might have a higher affinity for cyclophilin. This specific intra-mitochondrial distribution of the two ANT isotypes and cyclophilin D suggests specific functions of the peripheral and crista-forming parts of the inner membrane and the two ANT isotypes therein. IS - Pt 2 JF - Biochem J SN - 0264-6021 AD - A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, 119899, Russia. EP - 358 TI - Adenine nucleotide translocator isoforms 1 and 2 are differently distributed in the mitochondrial inner membrane and have distinct affinities to cyclophilin D. VL - 358 SP - 349 KW - ant KW - mitochondria AU - Vyssokikh, MY AU - Katz, A AU - Rueck, A AU - Wuensch, C AU - Dörner, A AU - Zorov, DB AU - Brdiczka, D PY - 2001/09/01/ UR - http://view.ncbi.nlm.nih.gov/pubmed/11513733 ER -