The protein cofactor necessary for ADP-ribosylation of Gs by cholera toxin is itself a GTP binding protein. Export

@article{citeulike:3041123, abstract = {A membrane-bound protein cofactor (ARF) is required for the cholera toxin-dependent ADP-ribosylation of the stimulatory regulatory component (Gs) of adenylate cyclase. Improved methods for the purification of ARF from bovine brain are described. ARF has a high-affinity binding site for guanine nucleotides. Binding of GTP or GTP gamma S to ARF is necessary for the activity of the cofactor; GDP X ARF does not support ADP-ribosylation of Gs. Although the protein as purified contains stoichiometric amounts of GDP, GTPase activity of isolated ARF was not detected. Cholera toxin-dependent activation of adenylate cyclase thus requires two guanine nucleotide binding proteins.}, author = {Kahn, R. A. and Gilman, A. G.}, citeulike-article-id = {3041123}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/3086320}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=3086320}, day = {15}, issn = {0021-9258}, journal = {The Journal of biological chemistry}, keywords = {arf, cholera}, month = {June}, number = {17}, pages = {7906--7911}, posted-at = {2008-07-24 20:48:37}, priority = {2}, title = {The protein cofactor necessary for ADP-ribosylation of Gs by cholera toxin is itself a GTP binding protein.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/3086320}, volume = {261}, year = {1986} }

TY - JOUR ID - citeulike:3041123 L3 - citeulike-article-id:3041123 N2 - A membrane-bound protein cofactor (ARF) is required for the cholera toxin-dependent ADP-ribosylation of the stimulatory regulatory component (Gs) of adenylate cyclase. Improved methods for the purification of ARF from bovine brain are described. ARF has a high-affinity binding site for guanine nucleotides. Binding of GTP or GTP gamma S to ARF is necessary for the activity of the cofactor; GDP X ARF does not support ADP-ribosylation of Gs. Although the protein as purified contains stoichiometric amounts of GDP, GTPase activity of isolated ARF was not detected. Cholera toxin-dependent activation of adenylate cyclase thus requires two guanine nucleotide binding proteins. IS - 17 JF - The Journal of biological chemistry SN - 0021-9258 EP - 7911 TI - The protein cofactor necessary for ADP-ribosylation of Gs by cholera toxin is itself a GTP binding protein. VL - 261 SP - 7906 KW - arf KW - cholera AU - Kahn, RA AU - Gilman, AG PY - 1986/06/15/ UR - http://view.ncbi.nlm.nih.gov/pubmed/3086320 ER -