Centriolin anchoring of exocyst and SNARE complexes at the midbody is required for secretory-vesicle-mediated abscission. Export

@article{citeulike:3883797, abstract = {The terminal step in cytokinesis, called abscission, requires resolution of the membrane connection between two prospective daughter cells. Our previous studies demonstrated that the coiled-coil protein centriolin localized to the midbody during cytokinesis and was required for abscission. Here we show that centriolin interacts with proteins of vesicle-targeting exocyst complexes and vesicle-fusion SNARE complexes. These complexes require centriolin for localization to a unique midbody-ring structure, and disruption of either complex inhibits abscission. Exocyst disruption induces accumulation of v-SNARE-containing vesicles at the midbody ring. In control cells, these v-SNARE vesicles colocalize with a GFP-tagged secreted polypeptide. The vesicles move to the midbody ring asymmetrically from one prospective daughter cell; the GFP signal is rapidly lost, suggesting membrane fusion; and subsequently the cell cleaves at the site of vesicle delivery/fusion. We propose that centriolin anchors protein complexes required for vesicle targeting and fusion and integrates membrane-vesicle fusion with abscission.}, author = {Gromley, A. and Yeaman, C. and Rosa, J. and Redick, S. and Chen, C. T. and Mirabelle, S. and Guha, M. and Sillibourne, J. and Doxsey, S. J.}, citeulike-article-id = {3883797}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.cell.2005.07.027}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16213214}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16213214}, day = {7}, doi = {10.1016/j.cell.2005.07.027}, issn = {0092-8674}, journal = {Cell}, keywords = {arl3, golgi}, month = {October}, number = {1}, pages = {75--87}, posted-at = {2009-01-13 22:02:46}, priority = {2}, title = {Centriolin anchoring of exocyst and SNARE complexes at the midbody is required for secretory-vesicle-mediated abscission.}, url = {http://dx.doi.org/10.1016/j.cell.2005.07.027}, volume = {123}, year = {2005} }

TY - JOUR ID - citeulike:3883797 L3 - citeulike-article-id:3883797 N2 - The terminal step in cytokinesis, called abscission, requires resolution of the membrane connection between two prospective daughter cells. Our previous studies demonstrated that the coiled-coil protein centriolin localized to the midbody during cytokinesis and was required for abscission. Here we show that centriolin interacts with proteins of vesicle-targeting exocyst complexes and vesicle-fusion SNARE complexes. These complexes require centriolin for localization to a unique midbody-ring structure, and disruption of either complex inhibits abscission. Exocyst disruption induces accumulation of v-SNARE-containing vesicles at the midbody ring. In control cells, these v-SNARE vesicles colocalize with a GFP-tagged secreted polypeptide. The vesicles move to the midbody ring asymmetrically from one prospective daughter cell; the GFP signal is rapidly lost, suggesting membrane fusion; and subsequently the cell cleaves at the site of vesicle delivery/fusion. We propose that centriolin anchors protein complexes required for vesicle targeting and fusion and integrates membrane-vesicle fusion with abscission. IS - 1 JF - Cell SN - 0092-8674 EP - 87 TI - Centriolin anchoring of exocyst and SNARE complexes at the midbody is required for secretory-vesicle-mediated abscission. VL - 123 SP - 75 KW - arl3 KW - golgi AU - Gromley, A AU - Yeaman, C AU - Rosa, J AU - Redick, S AU - Chen, CT AU - Mirabelle, S AU - Guha, M AU - Sillibourne, J AU - Doxsey, SJ PY - 2005/10/07/ UR - http://dx.doi.org/10.1016/j.cell.2005.07.027 ER -