Expression and evaluation of RING finger proteins. Export

@article{citeulike:510689, abstract = {RING finger proteins represent the largest class of potential ubiquitin ligases. This chapter describes methods used to express and assess the activity of proteins containing RING fingers based on our experience with a number of different family members. In addition to general protocols for assessing activity, specific protocols are provided for evaluating the ubiquitylation of p53 by the RING finger E3 Hdm2/Mdm2. Use of these methods may help identify new E3s, dissect factors involved in ubiquitylation of substrates, and screen for molecules that affect ubiquitylation.}, address = {Dynamics and Signaling, National Cancer Institute at Frederick, Frederick, MD 21702, USA.}, author = {Yang, Y. and Lorick, K. L. and Jensen, J. P. and Weissman, A. M.}, citeulike-article-id = {510689}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/S0076-6879(05)98010-5}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16275323}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16275323}, doi = {10.1016/S0076-6879(05)98010-5}, issn = {0076-6879}, journal = {Methods Enzymol}, keywords = {domain, ring}, pages = {103--112}, posted-at = {2006-02-18 18:11:03}, priority = {2}, title = {Expression and evaluation of RING finger proteins.}, url = {http://dx.doi.org/10.1016/S0076-6879(05)98010-5}, volume = {398}, year = {2005} }

TY - JOUR ID - citeulike:510689 L3 - citeulike-article-id:510689 N2 - RING finger proteins represent the largest class of potential ubiquitin ligases. This chapter describes methods used to express and assess the activity of proteins containing RING fingers based on our experience with a number of different family members. In addition to general protocols for assessing activity, specific protocols are provided for evaluating the ubiquitylation of p53 by the RING finger E3 Hdm2/Mdm2. Use of these methods may help identify new E3s, dissect factors involved in ubiquitylation of substrates, and screen for molecules that affect ubiquitylation. JF - Methods Enzymol SN - 0076-6879 AD - Dynamics and Signaling, National Cancer Institute at Frederick, Frederick, MD 21702, USA. EP - 112 TI - Expression and evaluation of RING finger proteins. VL - 398 SP - 103 KW - domain KW - ring AU - Yang, Y AU - Lorick, KL AU - Jensen, JP AU - Weissman, AM PY - 2005/// UR - http://dx.doi.org/10.1016/S0076-6879(05)98010-5 ER -