Localized Rac activation dynamics visualized in living cells. Export

@article{citeulike:925391, abstract = {Signaling proteins are thought to be tightly regulated spatially and temporally in order to generate specific and localized effects. For Rac and other small guanosine triphosphatases, binding to guanosine triphosphate leads to interaction with downstream targets and regulates subcellular localization. A method called FLAIR (fluorescence activation indicator for Rho proteins) was developed to quantify the spatio-temporal dynamics of the Rac1 nucleotide state in living cells. FLAIR revealed precise spatial control of growth factor-induced Rac activation, in membrane ruffles and in a gradient of activation at the leading edge of motile cells. FLAIR exemplifies a generally applicable approach for examining spatio-temporal control of protein activity.}, address = {Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.}, author = {Kraynov, V. S. and Chamberlain, C. and Bokoch, G. M. and Schwartz, M. A. and Slabaugh, S. and Hahn, K. M.}, citeulike-article-id = {925391}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.290.5490.333}, citeulike-linkout-1 = {http://www.sciencemag.org/cgi/content/abstract/290/5490/333}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/11030651}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=11030651}, day = {13}, doi = {10.1126/science.290.5490.333}, issn = {0036-8075}, journal = {Science}, keywords = {arl, fluorophore, methods}, month = {October}, number = {5490}, pages = {333--337}, posted-at = {2009-01-15 16:40:50}, priority = {2}, title = {Localized Rac activation dynamics visualized in living cells.}, url = {http://dx.doi.org/10.1126/science.290.5490.333}, volume = {290}, year = {2000} }

TY - JOUR ID - citeulike:925391 L3 - citeulike-article-id:925391 N2 - Signaling proteins are thought to be tightly regulated spatially and temporally in order to generate specific and localized effects. For Rac and other small guanosine triphosphatases, binding to guanosine triphosphate leads to interaction with downstream targets and regulates subcellular localization. A method called FLAIR (fluorescence activation indicator for Rho proteins) was developed to quantify the spatio-temporal dynamics of the Rac1 nucleotide state in living cells. FLAIR revealed precise spatial control of growth factor-induced Rac activation, in membrane ruffles and in a gradient of activation at the leading edge of motile cells. FLAIR exemplifies a generally applicable approach for examining spatio-temporal control of protein activity. IS - 5490 JF - Science SN - 0036-8075 AD - Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA. EP - 337 TI - Localized Rac activation dynamics visualized in living cells. VL - 290 SP - 333 KW - arl KW - fluorophore KW - methods AU - Kraynov, VS AU - Chamberlain, C AU - Bokoch, GM AU - Schwartz, MA AU - Slabaugh, S AU - Hahn, KM PY - 2000/10/13/ UR - http://dx.doi.org/10.1126/science.290.5490.333 ER -