Resolving vesicle fusion from lysis to monitor calcium-triggered lysosomal exocytosis in astrocytes. Export

@article{citeulike:3852369, abstract = {Optical imaging of individual vesicle exocytosis is providing new insights into the mechanism and regulation of secretion by cells. To study calcium-triggered secretion from astrocytes, we used acridine orange (AO) to label vesicles. Although AO is often used for imaging exocytosis, we found that imaging vesicles labeled with AO can result in their photolysis. Here, we define experimental and analytical approaches that permit us to distinguish unambiguously between fusion, leakage, and lysis of individual vesicles. We have used this approach to demonstrate that lysosomes undergo calcium-triggered exocytosis in astrocytes.}, author = {Jaiswal, J. K. and Fix, M. and Takano, T. and Nedergaard, M. and Simon, S. M.}, citeulike-article-id = {3852369}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0704935104}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17715060}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17715060}, doi = {10.1073/pnas.0704935104}, issn = {0027-8424}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {exocytosis, tirf}, month = {August}, number = {35}, pages = {14151--14156}, posted-at = {2009-01-06 10:46:19}, priority = {0}, title = {Resolving vesicle fusion from lysis to monitor calcium-triggered lysosomal exocytosis in astrocytes.}, url = {http://dx.doi.org/10.1073/pnas.0704935104}, volume = {104}, year = {2007} }

TY - JOUR ID - citeulike:3852369 L3 - citeulike-article-id:3852369 N2 - Optical imaging of individual vesicle exocytosis is providing new insights into the mechanism and regulation of secretion by cells. To study calcium-triggered secretion from astrocytes, we used acridine orange (AO) to label vesicles. Although AO is often used for imaging exocytosis, we found that imaging vesicles labeled with AO can result in their photolysis. Here, we define experimental and analytical approaches that permit us to distinguish unambiguously between fusion, leakage, and lysis of individual vesicles. We have used this approach to demonstrate that lysosomes undergo calcium-triggered exocytosis in astrocytes. IS - 35 JF - Proceedings of the National Academy of Sciences of the United States of America SN - 0027-8424 EP - 14156 TI - Resolving vesicle fusion from lysis to monitor calcium-triggered lysosomal exocytosis in astrocytes. VL - 104 SP - 14151 KW - exocytosis KW - tirf AU - Jaiswal, JK AU - Fix, M AU - Takano, T AU - Nedergaard, M AU - Simon, SM PY - 2007/08/28/ UR - http://dx.doi.org/10.1073/pnas.0704935104 ER -