The dynamic control of kiss-and-run and vesicular reuse probed with single nanoparticles Export

@article{19213879, abstract = {Vesicular secretion of neurotransmitter is essential for neuronal communication. Kiss-and-run is a mode of membrane fusion and retrieval without the full collapse of the vesicle into the plasma membrane and de novo regeneration. The importance of kiss-and-run during efficient neurotransmission has remained in doubt. We developed an approach for loading individual synaptic vesicles with single quantum dots. Their size and pH-dependent photoluminescence change allowed us to distinguish kiss-and-run from full-collapse fusion and to track single vesicles through multiple rounds of kiss-and-run and reuse, without perturbing vesicle cycling. Kiss-and-run dominated at the beginning of stimulus trains, reflecting the preference of vesicles with high release probability. Its incidence was increased by rapid firing, a response appropriate to shape the kinetics of neurotransmission during a wide range of firing patterns.}, address = {Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA.}, author = {Zhang, Q. and Li, Y. and Tsien, R. W.}, citeulike-article-id = {5378190}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1167373}, citeulike-linkout-1 = {http://www.sciencemag.org/cgi/content/full/323/5920/1448}, day = {13}, doi = {10.1126/science.1167373}, issn = {1095-9203}, journal = {Science}, keywords = {exocytosis, hippo-neurons-culture, quantum-dots, synaptic-cycle}, month = {March}, number = {5920}, pages = {1448--53}, pdf = {file://localhost/Users/christo/Travail/Labo/Biblio/Papers/Zhang\_Science\_2009.pdf}, posted-at = {2009-08-06 10:14:28}, priority = {0}, title = {The dynamic control of kiss-and-run and vesicular reuse probed with single nanoparticles}, url = {http://dx.doi.org/10.1126/science.1167373}, volume = {323}, year = {2009} }

TY - JOUR ID - 19213879 L3 - citeulike-article-id:5378190 N2 - Vesicular secretion of neurotransmitter is essential for neuronal communication. Kiss-and-run is a mode of membrane fusion and retrieval without the full collapse of the vesicle into the plasma membrane and de novo regeneration. The importance of kiss-and-run during efficient neurotransmission has remained in doubt. We developed an approach for loading individual synaptic vesicles with single quantum dots. Their size and pH-dependent photoluminescence change allowed us to distinguish kiss-and-run from full-collapse fusion and to track single vesicles through multiple rounds of kiss-and-run and reuse, without perturbing vesicle cycling. Kiss-and-run dominated at the beginning of stimulus trains, reflecting the preference of vesicles with high release probability. Its incidence was increased by rapid firing, a response appropriate to shape the kinetics of neurotransmission during a wide range of firing patterns. IS - 5920 JF - Science SN - 1095-9203 AD - Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA. EP - 53 TI - The dynamic control of kiss-and-run and vesicular reuse probed with single nanoparticles VL - 323 SP - 1448 KW - exocytosis KW - hippo-neurons-culture KW - quantum-dots KW - synaptic-cycle AU - Zhang, Q AU - Li, Y AU - Tsien, RW PY - 2009/03/13/ UR - http://dx.doi.org/10.1126/science.1167373 ER -