A functional barrier to movement of lipids in polarized neurons Export

@article{1406997, abstract = {In polarized neurons, axons and dendrites perform different functions, which are reflected in their different molecular organization. Studies on the sorting of viral and endogenous glycoproteins in epithelial cells and hippocampal neurons suggest that there may be similarities in the mechanism of sorting in these two cell types. The mechanisms that maintain the distinct composition of the two plasma membrane domains in these two cell types must, however, be different. We have proposed the existence of a functional barrier at the axonal hillock/initial segment which prevents the intermixing of membrane constituents. Here we test this hypothesis by fusing liposomes containing fluorescent phospholipids into the plasma membrane of polarized hippocampal cells in culture. Fusion was induced by lowering the pH and mediated by influenza virus haemagglutinin expressed on the axonal surface of neurons infected with fowl plague virus. Labelling was found exclusively on axons after fusion. Although the fused lipids were mobile on the axonal membrane, no labelling was detected on the cell body and dendritic surfaces. These results suggest that there is a diffusion barrier at the axonal hillock/initial segment which maintains the compositional differences between the axonal and somatodendritic domains.}, address = {Cell Biology Program, European Molecular Biology Laboratory, Heidelberg, Germany.}, author = {Kobayashi, T. and Storrie, B. and Simons, K. and Dotti, C. G.}, citeulike-article-id = {5378241}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/359647a0}, citeulike-linkout-1 = {http://www.nature.com/doifinder/10.1038/359647a0}, day = {15}, doi = {10.1038/359647a0}, issn = {0028-0836}, journal = {Nature}, keywords = {ais, hippo-neurons-culture, lipids, membrane-diffusion}, month = {October}, number = {6396}, pages = {647--50}, pdf = {file://localhost/Users/christo/Travail/Labo/Biblio/Papers/Kobayashi\_Nature\_1992.pdf}, posted-at = {2009-08-06 10:14:32}, priority = {0}, publisher = {Nature Publishing Group}, title = {A functional barrier to movement of lipids in polarized neurons}, url = {http://dx.doi.org/10.1038/359647a0}, volume = {359}, year = {1992} }

TY - JOUR ID - 1406997 L3 - citeulike-article-id:5378241 N2 - In polarized neurons, axons and dendrites perform different functions, which are reflected in their different molecular organization. Studies on the sorting of viral and endogenous glycoproteins in epithelial cells and hippocampal neurons suggest that there may be similarities in the mechanism of sorting in these two cell types. The mechanisms that maintain the distinct composition of the two plasma membrane domains in these two cell types must, however, be different. We have proposed the existence of a functional barrier at the axonal hillock/initial segment which prevents the intermixing of membrane constituents. Here we test this hypothesis by fusing liposomes containing fluorescent phospholipids into the plasma membrane of polarized hippocampal cells in culture. Fusion was induced by lowering the pH and mediated by influenza virus haemagglutinin expressed on the axonal surface of neurons infected with fowl plague virus. Labelling was found exclusively on axons after fusion. Although the fused lipids were mobile on the axonal membrane, no labelling was detected on the cell body and dendritic surfaces. These results suggest that there is a diffusion barrier at the axonal hillock/initial segment which maintains the compositional differences between the axonal and somatodendritic domains. IS - 6396 JF - Nature SN - 0028-0836 AD - Cell Biology Program, European Molecular Biology Laboratory, Heidelberg, Germany. EP - 50 TI - A functional barrier to movement of lipids in polarized neurons PB - Nature Publishing Group VL - 359 SP - 647 KW - ais KW - hippo-neurons-culture KW - lipids KW - membrane-diffusion AU - Kobayashi, T AU - Storrie, B AU - Simons, K AU - Dotti, CG PY - 1992/10/15/ UR - http://dx.doi.org/10.1038/359647a0 ER -