Defining mechanisms of actin polymerization and depolymerization during dendritic spine morphogenesis Export

@article{19380880, abstract = {Dendritic spines are small protrusions along dendrites where the postsynaptic components of most excitatory synapses reside in the mature brain. Morphological changes in these actin-rich structures are associated with learning and memory formation. Despite the pivotal role of the actin cytoskeleton in spine morphogenesis, little is known about the mechanisms regulating actin filament polymerization and depolymerization in dendritic spines. We show that the filopodia-like precursors of dendritic spines elongate through actin polymerization at both the filopodia tip and root. The small GTPase Rif and its effector mDia2 formin play a central role in regulating actin dynamics during filopodia elongation. Actin filament nucleation through the Arp2/3 complex subsequently promotes spine head expansion, and ADF/cofilin-induced actin filament disassembly is required to maintain proper spine length and morphology. Finally, we show that perturbation of these key steps in actin dynamics results in altered synaptic transmission.}, address = {Institute of Biotechnology, University of Helsinki, Helsinki FI-00014, Finland. pirta.hotulainen@helsinki.fi}, author = {Hotulainen, P. and Llano, O. and Smirnov, S. and Tanhuanp\"{a}\"{a}, K. and Faix, J. and Rivera, C. and Lappalainen, P.}, citeulike-article-id = {5378242}, citeulike-linkout-0 = {http://dx.doi.org/10.1083/jcb.200809046}, citeulike-linkout-1 = {http://jcb.rupress.org/cgi/pmidlookup?view=long&pmid=19380880}, day = {20}, doi = {10.1083/jcb.200809046}, issn = {0021-9525}, journal = {The Journal of Cell Biology}, keywords = {actin, cytosqueleton, dendrites, hippo-neurons-culture, spines}, month = {April}, number = {2}, pages = {323--39}, pdf = {file://localhost/Users/christo/Travail/Labo/Biblio/Papers/Hotulainen\_J\%20Cell\%20Biol\_2009.pdf}, posted-at = {2009-08-06 10:14:33}, priority = {0}, title = {Defining mechanisms of actin polymerization and depolymerization during dendritic spine morphogenesis}, url = {http://dx.doi.org/10.1083/jcb.200809046}, volume = {185}, year = {2009} }

TY - JOUR ID - 19380880 L3 - citeulike-article-id:5378242 N2 - Dendritic spines are small protrusions along dendrites where the postsynaptic components of most excitatory synapses reside in the mature brain. Morphological changes in these actin-rich structures are associated with learning and memory formation. Despite the pivotal role of the actin cytoskeleton in spine morphogenesis, little is known about the mechanisms regulating actin filament polymerization and depolymerization in dendritic spines. We show that the filopodia-like precursors of dendritic spines elongate through actin polymerization at both the filopodia tip and root. The small GTPase Rif and its effector mDia2 formin play a central role in regulating actin dynamics during filopodia elongation. Actin filament nucleation through the Arp2/3 complex subsequently promotes spine head expansion, and ADF/cofilin-induced actin filament disassembly is required to maintain proper spine length and morphology. Finally, we show that perturbation of these key steps in actin dynamics results in altered synaptic transmission. IS - 2 JF - The Journal of Cell Biology SN - 0021-9525 AD - Institute of Biotechnology, University of Helsinki, Helsinki FI-00014, Finland. pirta.hotulainen@helsinki.fi EP - 39 TI - Defining mechanisms of actin polymerization and depolymerization during dendritic spine morphogenesis VL - 185 SP - 323 KW - actin KW - cytosqueleton KW - dendrites KW - hippo-neurons-culture KW - spines AU - Hotulainen, P AU - Llano, O AU - Smirnov, S AU - Tanhuanpää, K AU - Faix, J AU - Rivera, C AU - Lappalainen, P PY - 2009/04/20/ UR - http://dx.doi.org/10.1083/jcb.200809046 ER -