Selective accumulation of virus-specific CD8+ T cells with unique homing phenotype within the human bone marrow Export

@article{citeulike:5971610, abstract = {The bone marrow plays a unique role within the immune system. We compared the phenotype and function of virus-specific CD8+ T cells from matched samples of human peripheral blood and bone marrow. Analysis of virus-specific memory CD8+ T cells showed widely divergent partition of antigen-specific populations between blood and bone marrow. T cells specific for Epstein-Barr virus (EBV) lytic antigens were enriched 3-fold in marrow compared with blood, whereas the response to EBV latent epitopes was equivalent between the 2 compartments. No difference in EBV viral load or expression of the EBV lytic protein was observed between blood and bone marrow. In direct contrast, although cytomegalo-virus (CMV)-specific T cells were the largest virus-specific population within peripheral blood, they were reduced by 60\% within marrow. Bone marrow T cells were found to exhibit a unique CCR5+CXCR6+CXCR3- homing phenotype which has not been observed on T cells from other secondary lymphoid organs or peripheral organs. Expression of CCR5 and CXCR6 was higher on EBV-specific T cells within peripheral blood compared with CMV-specific populations. These observations identify a novel bone marrow homing phenotype for CD8+ memory T cells, which necessitates a reevaluation of the magnitude of antigen-specific populations within the lymphoid system. 10.1182/blood-2008-02-138040}, author = {Palendira, Umaimainthan and Chinn, Rosanna and Raza, Wajid and Piper, Karen and Pratt, Guy and Machado, Lee and Bell, Andrew and Khan, Naeem and Hislop, Andrew D. and Steyn, Richard and Rickinson, Alan B. and Buckley, Christopher D. and Moss, Paul}, citeulike-article-id = {5971610}, citeulike-linkout-0 = {http://dx.doi.org/10.1182/blood-2008-02-138040}, citeulike-linkout-1 = {http://bloodjournal.hematologylibrary.org/content/112/8/3293.abstract}, citeulike-linkout-2 = {http://bloodjournal.hematologylibrary.org/content/112/8/3293.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/18635810}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=18635810}, day = {15}, doi = {10.1182/blood-2008-02-138040}, journal = {Blood}, keywords = {accumulation, antigen, antigens, blood\_and\_bone, ccr5, cd8\_t\_cells, direct\_contrast, epitopes, epstein\_barr\_virus, human\_bone\_marrow, immune\_system, lymphoid\_system, peripheral\_blood, peripheral\_organs, phenotype, reevaluation, secondary\_lymphoid\_organs, viral\_load, virus\_cmv, virus\_ebv}, month = {October}, number = {8}, pages = {3293--3302}, posted-at = {2009-10-19 17:25:02}, priority = {5}, title = {Selective accumulation of virus-specific CD8+ T cells with unique homing phenotype within the human bone marrow}, url = {http://dx.doi.org/10.1182/blood-2008-02-138040}, volume = {112}, year = {2008} }

TY - JOUR ID - citeulike:5971610 L3 - citeulike-article-id:5971610 N2 - The bone marrow plays a unique role within the immune system. We compared the phenotype and function of virus-specific CD8+ T cells from matched samples of human peripheral blood and bone marrow. Analysis of virus-specific memory CD8+ T cells showed widely divergent partition of antigen-specific populations between blood and bone marrow. T cells specific for Epstein-Barr virus (EBV) lytic antigens were enriched 3-fold in marrow compared with blood, whereas the response to EBV latent epitopes was equivalent between the 2 compartments. No difference in EBV viral load or expression of the EBV lytic protein was observed between blood and bone marrow. In direct contrast, although cytomegalo-virus (CMV)-specific T cells were the largest virus-specific population within peripheral blood, they were reduced by 60% within marrow. Bone marrow T cells were found to exhibit a unique CCR5+CXCR6+CXCR3- homing phenotype which has not been observed on T cells from other secondary lymphoid organs or peripheral organs. Expression of CCR5 and CXCR6 was higher on EBV-specific T cells within peripheral blood compared with CMV-specific populations. These observations identify a novel bone marrow homing phenotype for CD8+ memory T cells, which necessitates a reevaluation of the magnitude of antigen-specific populations within the lymphoid system. 10.1182/blood-2008-02-138040 IS - 8 JF - Blood EP - 3302 TI - Selective accumulation of virus-specific CD8+ T cells with unique homing phenotype within the human bone marrow VL - 112 SP - 3293 KW - accumulation KW - antigen KW - antigens KW - blood_and_bone KW - ccr5 KW - cd8_t_cells KW - direct_contrast KW - epitopes KW - epstein_barr_virus KW - human_bone_marrow KW - immune_system KW - lymphoid_system KW - peripheral_blood KW - peripheral_organs KW - phenotype KW - reevaluation KW - secondary_lymphoid_organs KW - viral_load KW - virus_cmv KW - virus_ebv AU - Palendira, Umaimainthan AU - Chinn, Rosanna AU - Raza, Wajid AU - Piper, Karen AU - Pratt, Guy AU - Machado, Lee AU - Bell, Andrew AU - Khan, Naeem AU - Hislop, Andrew AU - Steyn, Richard AU - Rickinson, Alan AU - Buckley, Christopher AU - Moss, Paul PY - 2008/10/15/ UR - http://dx.doi.org/10.1182/blood-2008-02-138040 ER -